反馈回路在胚胎基因调控网络中数量众多,并对这些网络的进化提供了稳定的影响。

IF 4.1 2区 生物学 Q1 DEVELOPMENTAL BIOLOGY
Evodevo Pub Date : 2023-06-16 DOI:10.1186/s13227-023-00214-y
Abdull Jesus Massri, Brennan McDonald, Gregory A Wray, David R McClay
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引用次数: 1

摘要

两种海胆——Lytechinus variegatus (Lv)和Strongylocentrotus purpuratus (Sp)——的发育基因调控网络(dGRNs)尽管自共同祖先以来已有5000万年的历史,但仍保持着惊人的相似性。数以百计的转录因子的平行实验扰动与类似的结果支持这一结论。最近的一项scRNA-seq分析表明,Lv和Sp的dgrn中有几个基因的最早表达不同。在这里,我们对这两个物种的dgrn进行了仔细的重新分析,并密切关注首次表达的时间。我们发现,在两个物种中,对细胞命运规范至关重要的基因的初始表达发生在几个压缩的时间段内。以前未被识别的反馈电路是从暂时校正的dgrn中推断出来的。虽然这些反馈在各自grn中的位置不同,但物种之间的总体数量是相似的。我们发现了关键发育调控基因首次表达时间的几个显著差异;与第三种物种的比较表明,这些异时性可能是在胚胎细胞谱系和进化分支方面以公正的方式产生的。总之,这些结果表明,即使在高度保守的dgrn中,相互作用也可以进化,反馈回路可能缓冲关键调控基因表达中的异时性的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Feedback circuits are numerous in embryonic gene regulatory networks and offer a stabilizing influence on evolution of those networks.

Feedback circuits are numerous in embryonic gene regulatory networks and offer a stabilizing influence on evolution of those networks.

Feedback circuits are numerous in embryonic gene regulatory networks and offer a stabilizing influence on evolution of those networks.

Feedback circuits are numerous in embryonic gene regulatory networks and offer a stabilizing influence on evolution of those networks.

The developmental gene regulatory networks (dGRNs) of two sea urchin species, Lytechinus variegatus (Lv) and Strongylocentrotus purpuratus (Sp), have remained remarkably similar despite about 50 million years since a common ancestor. Hundreds of parallel experimental perturbations of transcription factors with similar outcomes support this conclusion. A recent scRNA-seq analysis suggested that the earliest expression of several genes within the dGRNs differs between Lv and Sp. Here, we present a careful reanalysis of the dGRNs in these two species, paying close attention to timing of first expression. We find that initial expression of genes critical for cell fate specification occurs during several compressed time periods in both species. Previously unrecognized feedback circuits are inferred from the temporally corrected dGRNs. Although many of these feedbacks differ in location within the respective GRNs, the overall number is similar between species. We identify several prominent differences in timing of first expression for key developmental regulatory genes; comparison with a third species indicates that these heterochronies likely originated in an unbiased manner with respect to embryonic cell lineage and evolutionary branch. Together, these results suggest that interactions can evolve even within highly conserved dGRNs and that feedback circuits may buffer the effects of heterochronies in the expression of key regulatory genes.

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来源期刊
Evodevo
Evodevo EVOLUTIONARY BIOLOGY-DEVELOPMENTAL BIOLOGY
CiteScore
7.50
自引率
0.00%
发文量
18
审稿时长
>12 weeks
期刊介绍: EvoDevo publishes articles on a broad range of topics associated with the translation of genotype to phenotype in a phylogenetic context. Understanding the history of life, the evolution of novelty and the generation of form, whether through embryogenesis, budding, or regeneration are amongst the greatest challenges in biology. We support the understanding of these processes through the many complementary approaches that characterize the field of evo-devo. The focus of the journal is on research that promotes understanding of the pattern and process of morphological evolution. All articles that fulfill this aim will be welcome, in particular: evolution of pattern; formation comparative gene function/expression; life history evolution; homology and character evolution; comparative genomics; phylogenetics and palaeontology
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