Livia V Bayer, Samantha Milano, Stephen K Formel, Harpreet Kaur, Rishi Ravichandran, Juan A Cambeiro, Lizaveta Slinko, Irina E Catrina, Diana P Bratu
{"title":"在果蝇早期卵发生过程中,Cup对oskar mRNA翻译抑制至关重要。","authors":"Livia V Bayer, Samantha Milano, Stephen K Formel, Harpreet Kaur, Rishi Ravichandran, Juan A Cambeiro, Lizaveta Slinko, Irina E Catrina, Diana P Bratu","doi":"10.1080/15476286.2023.2242650","DOIUrl":null,"url":null,"abstract":"<p><p>Study of the timing and location for mRNA translation across model systems has begun to shed light on molecular events fundamental to such processes as intercellular communication, morphogenesis, and body pattern formation. In <i>D. melanogaster</i>, the posterior mRNA determinant, <i>oskar</i>, is transcribed maternally but translated only when properly localized at the oocyte's posterior cortex. Two effector proteins, Bruno1 and Cup, mediate steps of <i>oskar</i> mRNA regulation. The current model in the field identifies Bruno1 as necessary for Cup's recruitment to <i>oskar</i> mRNA and indispensable for <i>oskar</i>'s translational repression. We now report that this Bruno1-Cup interaction leads to precise <i>oskar</i> mRNA regulation during early oogenesis and, importantly, the two proteins mutually influence each other's mRNA expression and protein distribution in the egg chamber. We show that these factors stably associate with <i>oskar</i> mRNA <i>in vivo</i>. Cup associates with <i>oskar</i> mRNA without Bruno1, while surprisingly Bruno1's stable association with <i>oskar</i> mRNA depends on Cup. We demonstrate that the essential factor for <i>oskar</i> mRNA repression in early oogenesis is Cup, not Bruno1. Furthermore, we find that Cup is a key P-body component that maintains functional P-body morphology during oogenesis and is necessary for <i>oskar</i> mRNA's association with P-bodies. Therefore, Cup drives the translational repression and stability of <i>oskar</i> mRNA. These experimental results point to a regulatory feedback loop between Bruno 1 and Cup in early oogenesis that appears crucial for <i>oskar</i> mRNA to reach the posterior pole and its expression in the egg chamber for accurate embryo development.</p>","PeriodicalId":21351,"journal":{"name":"RNA Biology","volume":null,"pages":null},"PeriodicalIF":3.6000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/84/e1/KRNB_20_2242650.PMC10413924.pdf","citationCount":"0","resultStr":"{\"title\":\"Cup is essential for <i>oskar</i> mRNA translational repression during early <i>Drosophila</i> oogenesis.\",\"authors\":\"Livia V Bayer, Samantha Milano, Stephen K Formel, Harpreet Kaur, Rishi Ravichandran, Juan A Cambeiro, Lizaveta Slinko, Irina E Catrina, Diana P Bratu\",\"doi\":\"10.1080/15476286.2023.2242650\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Study of the timing and location for mRNA translation across model systems has begun to shed light on molecular events fundamental to such processes as intercellular communication, morphogenesis, and body pattern formation. In <i>D. melanogaster</i>, the posterior mRNA determinant, <i>oskar</i>, is transcribed maternally but translated only when properly localized at the oocyte's posterior cortex. Two effector proteins, Bruno1 and Cup, mediate steps of <i>oskar</i> mRNA regulation. The current model in the field identifies Bruno1 as necessary for Cup's recruitment to <i>oskar</i> mRNA and indispensable for <i>oskar</i>'s translational repression. We now report that this Bruno1-Cup interaction leads to precise <i>oskar</i> mRNA regulation during early oogenesis and, importantly, the two proteins mutually influence each other's mRNA expression and protein distribution in the egg chamber. We show that these factors stably associate with <i>oskar</i> mRNA <i>in vivo</i>. Cup associates with <i>oskar</i> mRNA without Bruno1, while surprisingly Bruno1's stable association with <i>oskar</i> mRNA depends on Cup. We demonstrate that the essential factor for <i>oskar</i> mRNA repression in early oogenesis is Cup, not Bruno1. Furthermore, we find that Cup is a key P-body component that maintains functional P-body morphology during oogenesis and is necessary for <i>oskar</i> mRNA's association with P-bodies. Therefore, Cup drives the translational repression and stability of <i>oskar</i> mRNA. These experimental results point to a regulatory feedback loop between Bruno 1 and Cup in early oogenesis that appears crucial for <i>oskar</i> mRNA to reach the posterior pole and its expression in the egg chamber for accurate embryo development.</p>\",\"PeriodicalId\":21351,\"journal\":{\"name\":\"RNA Biology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.6000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/84/e1/KRNB_20_2242650.PMC10413924.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"RNA Biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1080/15476286.2023.2242650\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"RNA Biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/15476286.2023.2242650","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Cup is essential for oskar mRNA translational repression during early Drosophila oogenesis.
Study of the timing and location for mRNA translation across model systems has begun to shed light on molecular events fundamental to such processes as intercellular communication, morphogenesis, and body pattern formation. In D. melanogaster, the posterior mRNA determinant, oskar, is transcribed maternally but translated only when properly localized at the oocyte's posterior cortex. Two effector proteins, Bruno1 and Cup, mediate steps of oskar mRNA regulation. The current model in the field identifies Bruno1 as necessary for Cup's recruitment to oskar mRNA and indispensable for oskar's translational repression. We now report that this Bruno1-Cup interaction leads to precise oskar mRNA regulation during early oogenesis and, importantly, the two proteins mutually influence each other's mRNA expression and protein distribution in the egg chamber. We show that these factors stably associate with oskar mRNA in vivo. Cup associates with oskar mRNA without Bruno1, while surprisingly Bruno1's stable association with oskar mRNA depends on Cup. We demonstrate that the essential factor for oskar mRNA repression in early oogenesis is Cup, not Bruno1. Furthermore, we find that Cup is a key P-body component that maintains functional P-body morphology during oogenesis and is necessary for oskar mRNA's association with P-bodies. Therefore, Cup drives the translational repression and stability of oskar mRNA. These experimental results point to a regulatory feedback loop between Bruno 1 and Cup in early oogenesis that appears crucial for oskar mRNA to reach the posterior pole and its expression in the egg chamber for accurate embryo development.
期刊介绍:
RNA has played a central role in all cellular processes since the beginning of life: decoding the genome, regulating gene expression, mediating molecular interactions, catalyzing chemical reactions. RNA Biology, as a leading journal in the field, provides a platform for presenting and discussing cutting-edge RNA research.
RNA Biology brings together a multidisciplinary community of scientists working in the areas of:
Transcription and splicing
Post-transcriptional regulation of gene expression
Non-coding RNAs
RNA localization
Translation and catalysis by RNA
Structural biology
Bioinformatics
RNA in disease and therapy