Yong-Jun Kang, Sang-Hyun Han, Sang-Geum Kim, Su-Yeon Kim, Hyeon-Ah Kim, Yoo-Kyung Kim, Ji-Hyun Yoo, Moon-Cheol Shin, Byoung-Chul Yang, Hee-Bok Park, Jun Heon Lee, In-Cheol Cho
{"title":"猪肌球蛋白重链3基因功能序列变异与肌肉胶原含量的关系。","authors":"Yong-Jun Kang, Sang-Hyun Han, Sang-Geum Kim, Su-Yeon Kim, Hyeon-Ah Kim, Yoo-Kyung Kim, Ji-Hyun Yoo, Moon-Cheol Shin, Byoung-Chul Yang, Hee-Bok Park, Jun Heon Lee, In-Cheol Cho","doi":"10.5187/jast.2023.e4","DOIUrl":null,"url":null,"abstract":"<p><p>This study examined the association between functional sequence variants (FSVs) of <i>myosin heavy chain 3</i> (<i>MYH3</i>) genotypes and collagen content in a Landrace and Jeju native pig (JNP) crossbred population. Four muscles (<i>Musculus longissimus dorsi</i>, <i>Musculus semimembranosus</i>, <i>Musculus triceps brachii</i>, and <i>Musculus</i> <i>biceps femoris</i>) were used for the analysis of meat collagen content, and the same animals were genotyped for the FSVs of the <i>MYH3</i> gene by using PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism). Three FSVs of <i>MYH3</i> genotypes were identified and had genotype frequencies of 0.358, 0.551, and 0.091 for <i>QQ</i>, <i>Qq</i>, and <i>qq</i>, respectively. <i>QQ</i> animals for the FSVs of the <i>MYH3</i> genotypes showed higher collagen content in their <i>M</i><i>. longissimus dorsi</i> (<i>p</i> < 0.001), <i>M. semimembranosus</i> (<i>p</i> < 0.001), <i>M. triceps brachii</i> (<i>p</i> < 0.001), and <i>M. biceps femoris</i> (<i>p</i> < 0.001) than <i>qq</i> homozygous animals. After the validation of this result in other independent populations, the FSVs of MYH3 genotypes can be a valuable genetic marker for improving collagen content in porcine muscles and can also be applied to increase the amount of collagen for biomedical purposes.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"65 3","pages":"511-518"},"PeriodicalIF":2.7000,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6d/41/jast-65-3-511.PMC10271922.pdf","citationCount":"1","resultStr":"{\"title\":\"Association of functional sequence variants of the <i>myosin heavy chain 3</i> gene with muscle collagen content in pigs.\",\"authors\":\"Yong-Jun Kang, Sang-Hyun Han, Sang-Geum Kim, Su-Yeon Kim, Hyeon-Ah Kim, Yoo-Kyung Kim, Ji-Hyun Yoo, Moon-Cheol Shin, Byoung-Chul Yang, Hee-Bok Park, Jun Heon Lee, In-Cheol Cho\",\"doi\":\"10.5187/jast.2023.e4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study examined the association between functional sequence variants (FSVs) of <i>myosin heavy chain 3</i> (<i>MYH3</i>) genotypes and collagen content in a Landrace and Jeju native pig (JNP) crossbred population. Four muscles (<i>Musculus longissimus dorsi</i>, <i>Musculus semimembranosus</i>, <i>Musculus triceps brachii</i>, and <i>Musculus</i> <i>biceps femoris</i>) were used for the analysis of meat collagen content, and the same animals were genotyped for the FSVs of the <i>MYH3</i> gene by using PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism). Three FSVs of <i>MYH3</i> genotypes were identified and had genotype frequencies of 0.358, 0.551, and 0.091 for <i>QQ</i>, <i>Qq</i>, and <i>qq</i>, respectively. <i>QQ</i> animals for the FSVs of the <i>MYH3</i> genotypes showed higher collagen content in their <i>M</i><i>. longissimus dorsi</i> (<i>p</i> < 0.001), <i>M. semimembranosus</i> (<i>p</i> < 0.001), <i>M. triceps brachii</i> (<i>p</i> < 0.001), and <i>M. biceps femoris</i> (<i>p</i> < 0.001) than <i>qq</i> homozygous animals. After the validation of this result in other independent populations, the FSVs of MYH3 genotypes can be a valuable genetic marker for improving collagen content in porcine muscles and can also be applied to increase the amount of collagen for biomedical purposes.</p>\",\"PeriodicalId\":14923,\"journal\":{\"name\":\"Journal of Animal Science and Technology\",\"volume\":\"65 3\",\"pages\":\"511-518\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2023-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6d/41/jast-65-3-511.PMC10271922.pdf\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Animal Science and Technology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.5187/jast.2023.e4\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Animal Science and Technology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.5187/jast.2023.e4","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Association of functional sequence variants of the myosin heavy chain 3 gene with muscle collagen content in pigs.
This study examined the association between functional sequence variants (FSVs) of myosin heavy chain 3 (MYH3) genotypes and collagen content in a Landrace and Jeju native pig (JNP) crossbred population. Four muscles (Musculus longissimus dorsi, Musculus semimembranosus, Musculus triceps brachii, and Musculusbiceps femoris) were used for the analysis of meat collagen content, and the same animals were genotyped for the FSVs of the MYH3 gene by using PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism). Three FSVs of MYH3 genotypes were identified and had genotype frequencies of 0.358, 0.551, and 0.091 for QQ, Qq, and qq, respectively. QQ animals for the FSVs of the MYH3 genotypes showed higher collagen content in their M. longissimus dorsi (p < 0.001), M. semimembranosus (p < 0.001), M. triceps brachii (p < 0.001), and M. biceps femoris (p < 0.001) than qq homozygous animals. After the validation of this result in other independent populations, the FSVs of MYH3 genotypes can be a valuable genetic marker for improving collagen content in porcine muscles and can also be applied to increase the amount of collagen for biomedical purposes.
期刊介绍：
Journal of Animal Science and Technology (J. Anim. Sci. Technol. or JAST) is a peer-reviewed, open access journal publishing original research, review articles and notes in all fields of animal science.
Topics covered by the journal include: genetics and breeding, physiology, nutrition of monogastric animals, nutrition of ruminants, animal products (milk, meat, eggs and their by-products) and their processing, grasslands and roughages, livestock environment, animal biotechnology, animal behavior and welfare.
Articles generally report research involving beef cattle, dairy cattle, pigs, companion animals, goats, horses, and sheep. However, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will also be considered for publication.
The Journal of Animal Science and Technology (J. Anim. Technol. or JAST) has been the official journal of The Korean Society of Animal Science and Technology (KSAST) since 2000, formerly known as The Korean Journal of Animal Sciences (launched in 1956).
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