与单独治疗相比,联合应用光生物调节和姜黄素负载氧化铁纳米颗粒显著增强了糖尿病大鼠感染、延迟修复伤口模型的修复。

IF 3.2 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Hamidreza Omidi, Kaysan Sohrabi, Abdollah Amini, Fatemeh Fadaei Fathabady, Atarodalsadat Mostafavinia, Houssein Ahmadi, Mansooreh Mirzaei, Fahimeh Ghasemi Moravej, Mohammadali Asghari, Fatemehalsadat Rezaei, Latif Gachkar, Sufan Chien, Mohammad Bayat
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引用次数: 2

摘要

在此,我们试图评估光生物调节剂(PBM)和姜黄素负载的铁纳米颗粒(CUR)单独或联合使用对I型糖尿病(TIDM)大鼠缺血感染延迟愈合伤口模型(IIDHWM)伤口愈合成熟阶段伤口愈合愈合率(WCR)、微生物菌群(通过测量菌落形成单位(cfu))、修复伤口的体力学和生物力学特性的治疗潜力。分为四组:1组为对照组,2组为CUR, 3组为PBM (80 Hz, 890 nm, 0.2 J/cm2), 4组为PBM和CUR (PBM + CUR)。我们发现,与1组相比,2组、3组和4组的CFU降低(p = 0.000)。与第1组相比,第2、3和4组的WCR显著升高(p = 0.000)。在创面强度参数方面,与1组相比,2组、3组和4组的弯曲刚度和高应力负荷显著增加(p = 0.000)。体视学检查显示,与1组相比,2、3、4组中性粒细胞和巨噬细胞计数减少,成纤维细胞计数增加(p = 0.000)。血管计数在PBM和PBM + CUR组中比1组更占优势(p = 0.000)。与CUR组相比,PBM + CUR组和PBM组的CFU和创面强度以及巨噬细胞、中性粒细胞和成纤维细胞计数均有所改善(p = 0.000 ~ p
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Application of combined photobiomodulation and curcumin-loaded iron oxide nanoparticles considerably enhanced repair in an infected, delayed-repair wound model in diabetic rats compared to either treatment alone.

Herein, we attempted to evaluate the therapeutic potential of photobiomodulation (PBM) and curcumin-loaded iron nanoparticles (CUR), alone and in combination, on wound closure rate (WCR), microbial flora by measuring colony-forming units (CFUs), the stereological and biomechanical properties of repairing wounds in the maturation stage of the wound healing course in an ischemic infected delayed healing wound model (IIDHWM) of type I diabetic (TIDM) rats. There were four groups: group 1 was the control, group 2 received CUR, rats in group 3 were exposed to PBM (80 Hz, 890 nm, and 0.2 J/cm2), and rats in group 4 received both PBM and CUR (PBM + CUR). We found CFU was decreased in groups 2, 3, and 4 compared to group 1 (p = 0.000 for all). Groups 2, 3, and 4 showed a considerable escalation in WCR compared to group 1 (p = 0.000 for all). In terms of wound strength parameters, substantial increases in bending stiffness and high-stress load were observed in groups 2, 3, and 4 compared to group 1 (p = 0.000 for all). Stereological examinations revealed decreases in neutrophil and macrophage counts and increases in fibroblast counts in groups 2, 3, and 4compared  to group 1 (p = 0.000 for all). Blood vessel counts were more dominant in the PBM and PBM + CUR groups over group 1 (p = 0.000 for all). CFU and wound strength as well as macrophage, neutrophil, and fibroblast counts were found to be improved in the PBM + CUR and PBM groups compared to the CUR group (ranging from p = 0.000 to p < 0.05). Better results were achieved in the PBM + CUR  treatment  over the PBM therapy. We determined therapy with PBM + CUR, PBM alone, and CUR alone substantially accelerated diabetic wound healing in an IIDHWM of TIDM rats compared to control  group. Concomitantly, the PBM + CUR and PBM groups attained significantly enhanced results for WCR, stereological parameters, and wound strength than the CUR group, with the PBM + CUR results being superior to those of the PBM group.

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来源期刊
Photochemical & Photobiological Sciences
Photochemical & Photobiological Sciences 生物-生化与分子生物学
CiteScore
5.60
自引率
6.50%
发文量
201
审稿时长
2.3 months
期刊介绍: A society-owned journal publishing high quality research on all aspects of photochemistry and photobiology.
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