酵母Sis1与Hsp70 c端EEVD肽复合物中残基1-81的主链和侧链NMR定位。

IF 0.8 4区生物学 Q4 BIOPHYSICS

Biomolecular NMR Assignments Pub Date : 2023-08-17 DOI:10.1007/s12104-023-10148-0

Carolina O. Matos, Glaucia M.S. Pinheiro, Carlos H. I. Ramos, Fabio C. L. Almeida

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引用次数: 0

摘要

分子伴侣有助于蛋白质折叠和组装，而不改变其最终结构，在几个折叠过程中，需要Hsp70和Hsp40家族成员之间的相互作用。在这里，我们报道了来自酿酒酵母Sis1的B类Hsp40的J结构域的主链和侧链的1H、15N和13C核与Hsp70的C末端EEVD基序复合的NMR化学位移分配。这些数据揭示了关于结构和骨架动力学的信息，这些信息大大增加了对J结构域-Hsp70-EEVD相互作用机制的理解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Backbone and sidechain NMR assignments of residues 1–81 from yeast Sis1 in complex with an Hsp70 C-terminal EEVD peptide

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Backbone and sidechain NMR assignments of residues 1–81 from yeast Sis1 in complex with an Hsp70 C-terminal EEVD peptide

Molecular chaperones aid proteins to fold and assemble without modifying their final structure, requiring, in several folding processes, the interplay between members of the Hsp70 and Hsp40 families. Here, we report the NMR chemical shift assignments for ¹ H, ¹⁵ N, and ¹³ C nuclei of the backbone and side chains of the J-domain of the class B Hsp40 from Saccharomyces cerevisiae, Sis1, complexed with the C-terminal EEVD motif of Hsp70. The data revealed information on the structure and backbone dynamics that add significantly to the understanding of the J-domain-Hsp70-EEVD mechanism of interaction.

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来源期刊

Biomolecular NMR Assignments 生物-光谱学

CiteScore

1.70

自引率

11.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties. Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.