Chitooligosaccharide-catechin conjugate loaded liposome using different stabilising agents: characteristics, stability, and bioactivities.

IF 3 4区医学 Q2 CHEMISTRY, APPLIED

Journal of microencapsulation Pub Date : 2023-09-01 DOI:10.1080/02652048.2023.2209658

Ajay Mittal, Avtar Singh, Hui Hong, Soottawat Benjakul

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引用次数: 0

Abstract

Aim: To determine the optimum condition for preparing chitooligosaccharide-catechin conjugate (COS-CAT) liposomes using different stabilising agents.

Methods: COS-CAT liposomes (0.1-1%, w/v) were prepared using soy phosphatidylcholine (SPC) (50-200 mM) and glycerol or cholesterol (25-100 mg). Encapsulation efficiency (EE), loading capacity (LC), physicochemical characteristics, FTIR spectra, thermal stability, and structure of COS-CAT liposomes were assessed.

Results: COS-CAT loaded liposome stabilised by cholesterol (COS-CAT-CHO) showed higher stability as shown by the highest EE (76.81%) and LC (4.57%) and the lowest zeta potential (ZP) (-76.51 mV), polydispersity index (PDI) (0.2674) and releasing efficiency (RE) (53.54%) (p < 0.05). COS-CAT-CHO showed the highest retention and relative remaining bioactivities of COS-CAT under various conditions (p < 0.05). FTIR spectra revealed the interaction between the choline group of SPC and -OH groups of COS-CAT. Phase transition temperature of COS-CAT-CHO was shifted to 184 °C, which was higher than others (p < 0.05).

Conclusion: SPC and cholesterol-based liposome could be used as a promising vesicle for maintaining bioactivities of COS-CAT.

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使用不同稳定剂的壳寡糖-儿茶素偶联负载脂质体:特性、稳定性和生物活性。

目的:确定不同稳定剂制备壳寡糖儿茶素缀合物(COS-CAT)脂质体的最佳条件。方法:采用大豆磷脂酰胆碱(SPC) (50-200 mM)和甘油或胆固醇(25-100 mg)制备COS-CAT脂质体(0.1-1%，w/v)。对COS-CAT脂质体的包封效率(EE)、载药量(LC)、理化特性、红外光谱(FTIR)、热稳定性和结构进行了评价。结果:胆固醇稳定的载COS-CAT脂质体(COS-CAT- cho)具有较高的稳定性，其EE(76.81%)和LC(4.57%)最高，ZP (-76.51 mV)最低，多分散指数(PDI)为0.2674，释放效率(RE)为53.54% (p p p)。结论:SPC和胆固醇脂质体可作为维持COS-CAT生物活性的有前景的囊泡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of microencapsulation 工程技术-工程：化工

CiteScore

6.30

自引率

2.60%

发文量

审稿时长

3 months

期刊介绍： The Journal of Microencapsulation is a well-established, peer-reviewed journal dedicated to the publication of original research findings related to the preparation, properties and uses of individually encapsulated novel small particles, as well as significant improvements to tried-and-tested techniques relevant to micro and nano particles and their use in a wide variety of industrial, engineering, pharmaceutical, biotechnology and research applications. Its scope extends beyond conventional microcapsules to all other small particulate systems such as self assembling structures that involve preparative manipulation. The journal covers: Chemistry of encapsulation materials Physics of release through the capsule wall and/or desorption from carrier Techniques of preparation, content and storage Many uses to which microcapsules are put.