Lysophosphatidic acid stimulates rat uterine contraction in vitro.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-06-06 DOI:10.1262/jrd.2023-011

Satoshi Nagashima, Takuma Kimura, Ryota Terashima, Makoto Sugiyama, Keiichiro Kizaki, Mitsumori Kawaminami, Shiro Kurusu

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Abstract

Lysophosphatidic acid (LPA) has been implicated in the uterine endometrial functions of implantation and decidualization; however, not much is known about its myometrial contractile function. Herein we characterized the uterotonic effects of LPA in non-pregnant (estrus) and peri-parturient rats in vitro. LPA dose-dependently (0.01-10 μM) stimulated the amplitude and integral, but not the frequency, of the uterine strip contraction of estrous rats. The stimulatory effect of LPA was enhanced 1 day before parturition but was lost 1 day postpartum. LPA did not cause the de novo synthesis of prostaglandin (PG) F₂α but stimulated contractions cooperatively with the PG. LPA-induced contractions were significantly inhibited by an LPA1/2/3 antagonist in the uteri of estrous rats but not in term rats. This study characterized the uterotonic effect of a natural LPA that occurs at physiological concentrations, changes with reproductive states, and is independent of mediation by the newly synthesized PG.

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溶血磷脂酸刺激体外大鼠子宫收缩。

溶血磷脂酸(LPA)与子宫内膜着床和去胎化功能有关;然而，其肌层收缩功能尚不清楚。我们在体外研究了LPA对未怀孕(发情)和围分娩大鼠的子宫扩张作用。LPA剂量依赖性(0.01 ~ 10 μM)刺激发情大鼠子宫条形收缩的幅度和积分，但不影响频率。LPA的刺激作用在产前1天增强，产后1天减弱。LPA不引起前列腺素F2α的重新合成，但与PG协同刺激收缩，LPA1/2/3拮抗剂在发情大鼠子宫内明显抑制LPA诱导的收缩，而在足月大鼠子宫内无明显抑制作用。本研究描述了天然LPA在生理浓度下的子宫张力效应，随着生殖状态的变化而变化，并且不依赖于新合成的PG的介导。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.