Analysis of Epstein-Barr Virus Infection in Oral Potentially Malignant Disorders and Oral Cancer: A Cross-Sectional Study.

IF 1.4 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of International Society of Preventive and Community Dentistry Pub Date : 2023-05-01 DOI:10.4103/jispcd.JISPCD_235_22

Jintana Pankam, Puangwan Lapthanasupkul, Nakarin Kitkumthorn, Dulyapong Rungraungrayabkul, Boworn Klongnoi, Siribang-On Piboonniyom Khovidhunkit

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Abstract

Aims and objectives: The primary objective of this study is to determine the prevalence of Epstein-Barr virus (EBV) in oral potentially malignant disorders (OPMDs) and oral cancer (OC) in a group of Thais using polymerase chain reaction (PCR) and Epstein-Barr encoding regions (EBERs) in situ hybridization (ISH). The secondary objective is to investigate the risk factors of OC and the association between the presence of EBV and risk factors of OC/site of oral lesions.

Materials and methods: Sixty-one participants attending the screening project for OC and OPMDs at the Northeastern district hospitals of Thailand were recruited. Information related to risk factors and biopsy tissues for histopathological diagnosis was collected. Sixty-seven paraffin tissue blocks, including 52 OPMDs and 15 OC specimens, were investigated for EBV infection, using PCR analysis with latent membrane protein-1 (LMP-1) primer and EBERs ISH. Pearson's Chi-square or Fisher's exact test was used to analyze the differences in variables between participants with OPMDs and OC, as appropriate. The association between EBV infection and related risk factors was analyzed using logistic regression with a significant level at 0.05.

Results: Using PCR analysis, 8 of 67 specimens (11.94%) were positive for LMP-1. Three cases of OPMDs were positive for both LMP-1 PCR and EBERs ISH. Regarding risk factors of OC, the two most common risk factors were betel nut chewing (52.46%) and working in sunlight (42.62%). The habit of taking alcohol was significantly different between the OC and the OPMDs groups (p = 0.009). The association between LMP-1 and the lesion at the tongue was statistically significant, with odds ratio = 4.900 (95% confidence interval = 1.046-22.943; p = 0.044).

Conclusions: The prevalence of EBV infection in this group of participants was low. However, OPMDs at the tongue exhibited a significant association with EBV infection.

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查看原文本刊更多论文

eb病毒感染在口腔潜在恶性疾病和口腔癌中的分析:一项横断面研究。

目的和目的:本研究的主要目的是利用聚合酶链反应(PCR)和Epstein-Barr编码区(EBERs)原位杂交(ISH)技术，确定泰国人群口腔潜在恶性疾病(OPMDs)和口腔癌(OC)中eb病毒(EBV)的患病率。次要目的是调查卵巢癌的危险因素以及EBV的存在与卵巢癌/口腔病变部位的危险因素之间的关系。材料与方法:在泰国东北地区医院招募61名参加OC和opmd筛查项目的参与者。收集与危险因素和活检组织病理诊断相关的信息。采用潜伏膜蛋白-1 (latent membrane protein-1, LMP-1)引物和EBERs ISH进行PCR分析，对67个石蜡组织块(包括52个OPMDs和15个OC)进行EBV感染检测。使用Pearson卡方检验或Fisher精确检验来分析opmd和OC参与者之间变量的差异。采用logistic回归分析EBV感染与相关危险因素的相关性，显著水平为0.05。结果:67例标本中有8例(11.94%)LMP-1阳性。3例OPMDs的LMP-1 PCR和EBERs ISH均呈阳性。在OC的危险因素中，嚼槟榔(52.46%)和暴晒(42.62%)是最常见的两个危险因素。饮酒习惯在OC组和opmd组之间存在显著差异(p = 0.009)。LMP-1与舌部病变的相关性有统计学意义，优势比为4.900(95%可信区间为1.046 ~ 22.943;P = 0.044)。结论:这组参与者的EBV感染率很低。然而，舌头上的opmd与EBV感染有显著的关联。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of International Society of Preventive and Community Dentistry DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

2.50

自引率

0.00%

发文量

123

期刊介绍： It is a journal aimed for research, scientific facts and details covering all specialties of dentistry with a good determination for exploring and sharing the knowledge in the medical and dental fraternity. The scope is therefore huge covering almost all streams of dentistry - starting from original studies, systematic reviews, narrative reviews, very unique case reports. Journal scope is not limited to these subjects and is more wider covering all specialities of dentistry follows: -Preventive and Community dentistry (Dental public health)- Endodontics- Oral and maxillofacial pathology- Oral and maxillofacial radiology- Oral and maxillofacial surgery (also called oral surgery)- Orthodontics and dentofacial orthopedics- Periodontology (also called periodontics)- Pediatric dentistry (also called pedodontics)- Prosthodontics (also called prosthetic dentistry)- Oral medicine- Special needs dentistry (also called special care dentistry)- Oral Biology- Forensic odontology- Geriatric dentistry or Geriodontics- Preventive and Social Medicine (Public health)- Our journal appreciates research articles pertaining with advancement of dentistry, preventive and community dentistry including oral epidemiology, oral health services research, oral health education and promotion, behavioral sciences related to dentistry, dental jurisprudence, ethics and oral health, economics, and quality assessment, recent advances in preventive dentistry and community dentistry.