Modulation of inflammatory and oxidative stress biomarkers due to dexamethasone exposure in chicken splenocytes

IF 1.4 3区农林科学 Q4 IMMUNOLOGY

Veterinary immunology and immunopathology Pub Date : 2023-08-01 DOI:10.1016/j.vetimm.2023.110632

Sonu Ambwani , Rigzin Dolma , Raunak Sharma , Amandip Kaur , Himani Singh , Anamitra Ruj , Tanuj Kumar Ambwani

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引用次数: 1

Abstract

Dexamethasone (DEXA) is a potent corticosteroid, commonly used for treating inflammatory, hypersensitive and allergic conditions. It is administered to birds with tumours. Many studies were conducted on its immunosuppressive effects; however none of the similar study is available employing chicken splenocytes culture system. The present study was conducted to assess DEXA induced alterations in inflammatory and oxidative stress biomarkers in chicken splenocytes due to its in vitro exposure. The maximum non-cytotoxic dose (MNCD) was evaluated and was further used for conducting lymphocytes proliferation assay (LPA), antioxidant assays (lipid peroxidation, GSH, superoxide dismutase and nitric oxide assays) and assessment of mRNA levels of various genes (IL-1β, IL-6, IL-10, LITAF, iNOS, NF-κB1, Nrf-2, Caspase-3 and -9) through qPCR. The MNCD was determined to be 30 ng/ml in chicken splenocytes culture system. DEXA caused reduction in B and T lymphocytes proliferation indicating its immunosuppressive effects, however improved the antioxidant status of the exposed splenocytes. The expression levels of IL-1β, IL-6, iNOS, LITAF and NF-κB1 were significantly reduced while IL-10 was enhanced, which signify potent anti-inflammatory potential of DEXA. NF-κB is a major transcription factor that regulates genes responsible for both, innate and adaptive immune responses and elicits inflammation. The nuclear factor erythroid 2-related factor 2 (Nrf-2) level was found to be up-regulated. Nrf-2 plays important role in combating the oxidant stress and its increased expression could be the reason of improved antioxidant status of DEXA exposed cells. Present findings indicated that DEXA exhibited modulation in anti-inflammatory, immunomodulatory and antioxidant mediators in chicken splenocytes.

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地塞米松对鸡脾细胞炎症和氧化应激生物标志物的调节作用

地塞米松（DEXA）是一种强效皮质类固醇，通常用于治疗炎症、过敏和过敏性疾病。它用于患有肿瘤的鸟类。对其免疫抑制作用进行了许多研究；然而，使用鸡脾细胞培养系统的类似研究都不可用。本研究旨在评估由于体外暴露于DEXA，DEXA诱导的鸡脾细胞炎症和氧化应激生物标志物的变化。评估最大非细胞毒性剂量（MNCD），并通过qPCR进一步用于进行淋巴细胞增殖测定（LPA）、抗氧化测定（脂质过氧化、GSH、超氧化物歧化酶和一氧化氮测定）和评估各种基因（IL-1β、IL-6、IL-10、LITAF、iNOS、NF-κB1、Nrf-2、Caspase-3和-9）的mRNA水平。在鸡脾细胞培养系统中测定MNCD为30ng/ml。DEXA导致B和T淋巴细胞增殖减少，表明其免疫抑制作用，但改善了暴露的脾细胞的抗氧化状态。IL-1β、IL-6、iNOS、LITAF和NF-κB1的表达水平显著降低，而IL-10的表达水平升高，这表明DEXA具有强大的抗炎潜力。NF-κB是一种主要的转录因子，它调节负责先天和适应性免疫反应的基因，并引发炎症。发现核因子红系2相关因子2（Nrf-2）水平上调。Nrf-2在对抗氧化应激中起着重要作用，其表达增加可能是DEXA暴露细胞抗氧化状态改善的原因。目前的研究结果表明，DEXA对鸡脾细胞的抗炎、免疫调节和抗氧化介质具有调节作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary immunology and immunopathology 农林科学-免疫学

CiteScore

3.40

自引率

5.60%

发文量

审稿时长

70 days

期刊介绍： The journal reports basic, comparative and clinical immunology as they pertain to the animal species designated here: livestock, poultry, and fish species that are major food animals and companion animals such as cats, dogs, horses and camels, and wildlife species that act as reservoirs for food, companion or human infectious diseases, or as models for human disease. Rodent models of infectious diseases that are of importance in the animal species indicated above,when the disease requires a level of containment that is not readily available for larger animal experimentation (ABSL3), will be considered. Papers on rabbits, lizards, guinea pigs, badgers, armadillos, elephants, antelope, and buffalo will be reviewed if the research advances our fundamental understanding of immunology, or if they act as a reservoir of infectious disease for the primary animal species designated above, or for humans. Manuscripts employing other species will be reviewed if justified as fitting into the categories above. The following topics are appropriate: biology of cells and mechanisms of the immune system, immunochemistry, immunodeficiencies, immunodiagnosis, immunogenetics, immunopathology, immunology of infectious disease and tumors, immunoprophylaxis including vaccine development and delivery, immunological aspects of pregnancy including passive immunity, autoimmuity, neuroimmunology, and transplanatation immunology. Manuscripts that describe new genes and development of tools such as monoclonal antibodies are also of interest when part of a larger biological study. Studies employing extracts or constituents (plant extracts, feed additives or microbiome) must be sufficiently defined to be reproduced in other laboratories and also provide evidence for possible mechanisms and not simply show an effect on the immune system.