Electropolymerization processing of side-chain engineered EDOT for high performance microelectrode arrays

IF 10.5 1区 生物学 Q1 BIOPHYSICS
Mahdi Ghazal , Anna Susloparova , Camille Lefebvre , Michel Daher Mansour , Najami Ghodhbane , Alexis Melot , Corentin Scholaert , David Guérin , Sébastien Janel , Nicolas Barois , Morvane Colin , Luc Buée , Pierre Yger , Sophie Halliez , Yannick Coffinier , Sébastien Pecqueur , Fabien Alibart
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引用次数: 0

Abstract

Microelectrode Arrays (MEAs) are popular tools for in vitro extracellular recording. They are often optimized by surface engineering to improve affinity with neurons and guarantee higher recording quality and stability. Recently, PEDOT:PSS has been used to coat microelectrodes due to its good biocompatibility and low impedance, which enhances neural coupling. Herein, we investigate on electro-co-polymerization of EDOT with its triglymated derivative to control valence between monomer units and hydrophilic functions on a conducting polymer. Molecular packing, cation complexation, dopant stoichiometry are governed by the glycolation degree of the electro-active coating of the microelectrodes. Optimal monomer ratio allows fine-tuning the material hydrophilicity and biocompatibility without compromising the electrochemical impedance of microelectrodes nor their stability while interfaced with a neural cell culture. After incubation, sensing readout on the modified electrodes shows higher performances with respect to unmodified electropolymerized PEDOT, with higher signal-to-noise ratio (SNR) and higher spike counts on the same neural culture. Reported SNR values are superior to that of state-of-the-art PEDOT microelectrodes and close to that of state-of-the-art 3D microelectrodes, with a reduced fabrication complexity. Thanks to this versatile technique and its impact on the surface chemistry of the microelectrode, we show that electro-co-polymerization trades with many-compound properties to easily gather them into single macromolecular structures. Applied on sensor arrays, it holds great potential for the customization of neurosensors to adapt to environmental boundaries and to optimize extracted sensing features.

用于高性能微电极阵列的侧链工程EDOT电聚合工艺。
微电极阵列(MEA)是用于体外细胞外记录的流行工具。它们通常通过表面工程进行优化,以提高与神经元的亲和力,并保证更高的记录质量和稳定性。最近,PEDOT:PSS由于其良好的生物相容性和低阻抗,增强了神经耦合,已被用于涂覆微电极。在此,我们研究了EDOT与其三元配合衍生物的电共聚合,以控制导电聚合物上单体单元之间的价态和亲水功能。分子堆积、阳离子络合、掺杂剂化学计量由微电极电活性涂层的糖酵化度决定。最佳的单体比例允许微调材料的亲水性和生物相容性,而不会影响微电极的电化学阻抗及其与神经细胞培养物接触时的稳定性。孵育后,相对于未修饰的电聚合PEDOT,修饰电极上的传感读数显示出更高的性能,在相同的神经培养物上具有更高的信噪比(SNR)和更高的尖峰计数。报道的SNR值优于最先进的PEDOT微电极,接近最先进的3D微电极,制造复杂性降低。由于这种通用技术及其对微电极表面化学的影响,我们表明电共聚合具有许多化合物性质,可以很容易地将它们聚集成单个大分子结构。应用于传感器阵列,它在定制神经传感器以适应环境边界和优化提取的传感特征方面具有巨大的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biosensors and Bioelectronics
Biosensors and Bioelectronics 工程技术-电化学
CiteScore
20.80
自引率
7.10%
发文量
1006
审稿时长
29 days
期刊介绍: Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.
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