Comprehensive Immunohistochemical Analysis of Mesonephric Marker Expression in Low-grade Endometrial Endometrioid Carcinoma.

IF 1.6 4区 医学 Q3 OBSTETRICS & GYNECOLOGY
Yurimi Lee, Sangjoon Choi, Hyun-Soo Kim
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引用次数: 0

Abstract

Immunohistochemical markers shown to be useful in identifying/confirming mesonephric/mesonephric-like differentiation (MLD markers) include thyroid transcription factor (TTF1), GATA-binding protein 3 (GATA3), and cluster of differentiation 10 (CD10). Only a few studies have examined the expression levels of MLD markers in endometrial endometrioid carcinomas (EECs). This study aimed to analyze the frequency and pattern of MLD marker expression in low-grade EECs. We performed immunostaining for the detection of TTF1, GATA3, and CD10 expression in 50 low-grade EEC tissue samples and evaluated their staining proportion and intensity. Nine tumors (18.0%) expressed at least one MLD marker in varying proportions and intensities, and 2 of these tumors were positive for 2 MLD markers (TTF1/GATA3 and GATA3/CD10, respectively). Three (6.0%) tumors showed moderate-to-strong nuclear TTF1 immunoreactivity in ≤5% of the tumor cells. Five tumors (10.0%) had at least moderate nuclear GATA3 staining, and three of them displayed a staining proportion of ≥15%. Three tumors (6.0%) were focal (mean proportion, 15%) but strongly positive for CD10. Our findings indicate that a subset of EEC can express one or more MLD markers with varying staining proportions and intensities. Given that a diagnosis of uterine mesonephric-like adenocarcinoma should be established based on a combination of characteristic histologic features, unique immunophenotypes, and confirmed molecular findings, pathologists should not exclude EEC based only on the presence of focal immunoreactivity for MLD markers. Awareness of the atypical expression patterns of MLD markers in EEC helps pathologists avoid misdiagnosing EEC as a uterine mesonephric-like adenocarcinoma.

低级别子宫内膜样癌中中肾标记物表达的全面免疫组化分析
免疫组化标记物被证明有助于识别/确认间肾/中肾样分化(MLD标记物),包括甲状腺转录因子(TTF1)、GATA结合蛋白3(GATA3)和分化簇10(CD10)。只有少数研究检测了子宫内膜样癌(EECs)中MLD标记物的表达水平。本研究旨在分析 MLD 标记在低级别 EECs 中的表达频率和模式。我们对50个低分化EEC组织样本进行了免疫染色,检测TTF1、GATA3和CD10的表达,并评估了它们的染色比例和强度。九个肿瘤(18.0%)以不同的比例和强度表达了至少一种 MLD 标记,其中两个肿瘤的两种 MLD 标记(分别为 TTF1/GATA3 和 GATA3/CD10)均呈阳性。3个肿瘤(6.0%)在≤5%的肿瘤细胞中显示出中等至强的核TTF1免疫反应。5个肿瘤(10.0%)至少有中度的核GATA3染色,其中3个肿瘤的染色比例≥15%。三个肿瘤(6.0%)为局灶性(平均比例为 15%),但 CD10 强阳性。我们的研究结果表明,EEC 的一部分可表达一种或多种 MLD 标记,染色比例和强度各不相同。鉴于子宫系膜样腺癌的诊断应根据特征性组织学特征、独特的免疫表型和确证的分子研究结果综合确定,病理学家不应仅根据 MLD 标记的局灶性免疫反应来排除 EEC。认识到 MLD 标记物在 EEC 中的非典型表达模式有助于病理学家避免将 EEC 误诊为子宫间质样腺癌。
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来源期刊
CiteScore
3.90
自引率
12.50%
发文量
154
审稿时长
6-12 weeks
期刊介绍: International Journal of Gynecological Pathology is the official journal of the International Society of Gynecological Pathologists (ISGyP), and provides complete and timely coverage of advances in the understanding and management of gynecological disease. Emphasis is placed on investigations in the field of anatomic pathology. Articles devoted to experimental or animal pathology clearly relevant to an understanding of human disease are published, as are pathological and clinicopathological studies and individual case reports that offer new insights.
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