A HPLC-MS/MS method for the determination of Nadolol in rat plasma: Development, validation, and application to pharmacokinetic study.

Abstract

A sensitive validated method has been developed for the quantification of Nadolol in rat plasma by high performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) using deuterated Nadolol (Nadolol D9) as internal standard (IS). The liquid-liquid extraction method using ethyl acetate was employed for the sample pretreatment. The separation was achieved on the Agilent Zorbax XDB C18 column (150  mm  ×  4.6  mm ID., 3.5  μm). The column temperature was controlled at 30°C. The components were eluted by using mobile phase A (10  mM ammonium formate) and mobile phase B (acetonitrile) in the ratio of 20:80  v/v with a flow rate of 0.5  mL/min. And 15  μL aliquot was injected in an isocratic elution mode with a total run time of 2.5  min. The multiple reactions monitoring transitions, m/z 310.20/254.10 for Nadolol and IS 319.20/255.00 were selected to achieve high selective analysis. The method exhibited great selectivity and linearity over the concentration range of 6 to 3000  ng/mL. The lower limit of quantification was found to be 6  ng/mL. The developed method proved acceptable results on selectivity, sensitivity, precision, accuracy, and stability studies as per Food and Drug Administration guidelines. This HPLC-MS/MS assay was successfully applied to get the pharmacokinetics parameters in rat plasma.