Dissection of physiological, transcriptional, and metabolic traits in two tall fescue genotypes with contrasting drought tolerance.

Q3 Agricultural and Biological Sciences
Plant-environment interactions (Hoboken, N.J.) Pub Date : 2021-11-22 eCollection Date: 2021-12-01 DOI:10.1002/pei3.10066
Yun Kang, Shyamal Talukder, Zewei An, Ivone Torres-Jerez, Nick Krom, David Huhman, Michael Udvardi, Malay C Saha
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引用次数: 1

Abstract

Tall fescue (Festuca arundinacea) is an important cool-season perennial forage grass that forms mutualistic symbioses with fungal endophytes. Physiological, biochemical and transcriptional comparisons were made between two tall fescue genotypes with contrasting drought tolerance (tolerant, T400, and sensitive, S279), either with or without endophyte (Epichloë coenophiala). Drought stress was applied by withholding watering until plants reached mild, moderate and severe stresses. Physiological characterization showed that T400 had narrower, thicker leaves, and lower leaf conductance under well-watered conditions, compared to S279. After severe drought and recovery, endophytic T400 had greater shoot and root biomass than other plant types. Under drought, leaf osmotic pressure increased much more in T400 than S279, consistent with accumulation of metabolites/osmolytes, especially proline. Gene Ontology enrichment analysis indicated that T400 had more active organic acid metabolism than S279 under drought, and implicated the role of endophyte in stimulating protein metabolism in both genotypes. Overall T400 and S279 responded to endophyte differently in aspects of physiology, gene transcription and metabolites, indicating plant genotype-specific reactions to endophyte infection.

Abstract Image

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两个高羊茅基因型抗旱性对比的生理、转录和代谢特性的剖析。
高羊茅(Festuca arundinacea)是一种重要的冷季多年生牧草,与真菌内生菌形成共生关系。在具有或不具有内生菌(Epichloëcoenophiala)的两种耐旱性(耐受性,T400和敏感性,S279)的高羊茅基因型之间进行了生理、生化和转录比较。干旱胁迫是通过停止浇水直到植物达到轻度、中度和重度胁迫来施加的。生理特性表明,与S279相比,T400在良好的水分条件下具有更窄、更厚的叶片和更低的叶片电导。在严重干旱和恢复后,内生植物T400的地上部和根部生物量高于其他植物类型。在干旱条件下,T400的叶片渗透压比S279增加得更多,这与代谢物/渗透液的积累一致,尤其是脯氨酸。基因本体富集分析表明,在干旱条件下,T400比S279具有更活跃的有机酸代谢,并暗示了内生菌在两种基因型中刺激蛋白质代谢的作用。总体而言,T400和S279在生理学、基因转录和代谢产物方面对内生菌的反应不同,表明植物对内生真菌感染的基因型特异性反应。
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CiteScore
2.70
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审稿时长
15 weeks
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