Regulatory roles of lncRNA PANDAR in breast cancer cell proliferation.

IF 0.4 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Asian Biomedicine Pub Date : 2021-12-01 DOI:10.2478/abm-2021-0035

Qinnuan Sun, Xiumei Wang

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引用次数: 0

Abstract

Background: Breast cancer represents the second most deadly malignancy in women, and long noncoding RNAs (lncRNAs) have crucial functions in its development.

Objective: To investigate effects of the promoter of CDKN1A antisense DNA damage-activated RNA (PANDAR) on epithelial-mesenchymal transition (EMT) in breast cancer cells and their proliferation.

Methods: lncRNAs potentially regulating the transcriptional activity of the E-cadherin (E-cad, an epithelial cell marker) gene promoter were screened using a dual-luciferase reporter assay. PANDAR was overexpressed in Michigan cancer foundation 7 (MCF-7) breast cancer cells. E-cad and N-cadherin (N-cad, a mesenchymal cell marker) levels were detected by immunoblotting. Cell viability was assessed using a cell counting kit-8.

Results: PANDAR and TCONS00068220/LOC105375819 conservatively regulated the promoter activity of E-cad. PANDAR overexpression in MCF-7 inhibited E-cad expression, but upregulated N-cad. The enhanced expression of PANDAR promoted cell proliferation.

Conclusion: PANDAR is a key transcriptional repressor of E-cad and has regulatory effects on the promotion of cell proliferation. PANDAR is an oncogene in breast cancer, potentially facilitating the EMT process and promoting cell proliferation.

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lncRNA PANDAR在乳腺癌细胞增殖中的调控作用。

背景:乳腺癌是女性中第二致命的恶性肿瘤，长链非编码rna (lncRNAs)在其发展中起着至关重要的作用。目的:探讨CDKN1A反义DNA损伤激活RNA (PANDAR)启动子对乳腺癌细胞上皮-间质转化(EMT)及其增殖的影响。方法:使用双荧光素酶报告基因试验筛选可能调节E-cadherin(上皮细胞标记物E-cad)基因启动子转录活性的lncrna。PANDAR在密歇根癌症基金会7 (MCF-7)乳腺癌细胞中过表达。免疫印迹法检测E-cad和n -钙粘蛋白(N-cad，一种间充质细胞标志物)水平。使用细胞计数试剂盒-8评估细胞活力。结果:PANDAR和tcon00068220 /LOC105375819保守调控E-cad启动子活性。MCF-7中PANDAR的过表达抑制E-cad的表达，但上调N-cad的表达。PANDAR表达增强可促进细胞增殖。结论:PANDAR是E-cad的关键转录抑制因子，具有促进细胞增殖的调节作用。PANDAR是乳腺癌的致癌基因，可能促进EMT过程并促进细胞增殖。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Asian Biomedicine 医学-医学：研究与实验

CiteScore

1.20

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Asian Biomedicine: Research, Reviews and News (ISSN 1905-7415 print; 1875-855X online) is published in one volume (of 6 bimonthly issues) a year since 2007. [...]Asian Biomedicine is an international, general medical and biomedical journal that aims to publish original peer-reviewed contributions dealing with various topics in the biomedical and health sciences from basic experimental to clinical aspects. The work and authorship must be strongly affiliated with a country in Asia, or with specific importance and relevance to the Asian region. The Journal will publish reviews, original experimental studies, observational studies, technical and clinical (case) reports, practice guidelines, historical perspectives of Asian biomedicine, clinicopathological conferences, and commentaries Asian biomedicine is intended for a broad and international audience, primarily those in the health professions including researchers, physician practitioners, basic medical scientists, dentists, educators, administrators, those in the assistive professions, such as nurses, and the many types of allied health professionals in research and health care delivery systems including those in training.

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