The Effect of Training Type on The Signaling P athway of Ceramide-Dependent Insulin Resistance in The Flexor Hallucis Longus Muscle of Streptozotocin-Induced Diabetic Rats.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-07-25 DOI:10.22074/cellj.2023.1971874.1158

Pakhshan Rahimpour, Dariush Sheikholeslami-Vatani, Mohammad Raman Moloudi, Saeed Ghaeeni

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Abstract

Objective: This study aimed to compare the effect of different physical training on the mechanism of ceramidedependent insulin resistance in the flexor hallucis longus (FHL) muscle of diabetic rats.

Materials and methods: In this experimental study, 7 healthy as a healthy control (HC) group, and 21 diabetics (55 mg/ kg Streptozotocin) Wistar rats (200-220 g; 8-10 weeks old) divided into the diabetic control (DC), moderate continuous training (MCT), and moderate intensity interval training (MIIT) groups. Both MCT (55-70% of maximal oxygen uptake (VO_2max), and MIIT (85% VO_2max) groups trained for 10-25 minutes at a speed of 10-20 m/minutes. The changes in the expression of blood glucose, insulin, insulin resistance, lipid profile and total ceramide were measured as well as ceramide synthase-1, Glucose transporter type 4 (GLUT4), Protein kinase B known as Akt, phosphorylated protein kinase B known as pAkt, protein kinase C (PKC), and tumour necrosis factor α (TNFα).

Results: Blood glucose, triglyceride (TG) and ceramide synthase-1 (CS1) expression levels in the MCT group decreased in comparison with the DC group. FHL protein expression of GLUT4 in the MCT group was higher than the DC group. FHL expression of GLUT4, pAKT, AKT/pAKT, PKC, CS1 and total ceramide in the MIIT group were higher than the DC group. Cholesterol, low-density lipoprotein (LDL), TG, and TNF-α protein expression in the MIIT group were lower than the DC group. GLUT4, PKC, pAKT, AKT/pAKT in the MIIT group were higher, and total ceramide and TNF-α were lower in the MIIT group than the MCT group.

Conclusion: It seems that both training plan MIIT and MCT have favorable effects on the metabolism of glucose, insulin, lipids, and the decrease of TNFα level in the diabetes, but in connection with the improvement of the ceramides mechanism, it seems that the MIIT training plan is more optimal than MCT training plan.

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训练类型对链脲佐菌素诱导的糖尿病大鼠幻觉长屈肌神经酰胺依赖性胰岛素抵抗信号通路的影响。

目的:探讨不同运动方式对糖尿病大鼠幻觉长屈肌神经酰胺依赖性胰岛素抵抗的影响。材料与方法:本实验选用健康对照(HC)组7只，糖尿病组21只(链脲佐菌素55 mg/ kg) Wistar大鼠(200-220 g;8-10周龄)分为糖尿病控制组(DC)、中等强度连续训练组(MCT)和中等强度间歇训练组(MIIT)。MCT组(最大摄氧量55-70%)和MIIT组(最大摄氧量85%)以10-20米/分钟的速度训练10-25分钟。测定血糖、胰岛素、胰岛素抵抗、血脂和总神经酰胺的表达变化，以及神经酰胺合成酶-1、葡萄糖转运蛋白4型(GLUT4)、蛋白激酶B (Akt)、磷酸化蛋白激酶B (pAkt)、蛋白激酶C (PKC)和肿瘤坏死因子α (TNFα)的表达变化。结果:MCT组血糖、甘油三酯(TG)、神经酰胺合成酶-1 (CS1)表达水平较DC组降低。MCT组FHL蛋白GLUT4表达高于DC组。MIIT组FHL中GLUT4、pAKT、AKT/pAKT、PKC、CS1和总神经酰胺的表达均高于DC组。MIIT组胆固醇、低密度脂蛋白(LDL)、TG、TNF-α蛋白表达均低于DC组。MIIT组GLUT4、PKC、pAKT、AKT/pAKT均高于MCT组，总神经酰胺、TNF-α均低于MCT组。结论:MIIT训练计划和MCT训练计划似乎对糖尿病患者的葡萄糖、胰岛素、血脂代谢以及TNFα水平的降低都有良好的影响，但从神经酰胺机制的改善来看，MIIT训练计划似乎比MCT训练计划更优。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.