Transcription Factor Dynamics: One Molecule at a Time.

IF 11.4 1区 生物学 Q1 CELL BIOLOGY
Kaustubh Wagh, Diana A Stavreva, Arpita Upadhyaya, Gordon L Hager
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引用次数: 0

Abstract

Cells must tightly regulate their gene expression programs and yet rapidly respond to acute biochemical and biophysical cues within their environment. This information is transmitted to the nucleus through various signaling cascades, culminating in the activation or repression of target genes. Transcription factors (TFs) are key mediators of these signals, binding to specific regulatory elements within chromatin. While live-cell imaging has conclusively proven that TF-chromatin interactions are highly dynamic, how such transient interactions can have long-term impacts on developmental trajectories and disease progression is still largely unclear. In this review, we summarize our current understanding of the dynamic nature of TF functions, starting with a historical overview of early live-cell experiments. We highlight key factors that govern TF dynamics and how TF dynamics, in turn, affect downstream transcriptional bursting. Finally, we conclude with open challenges and emerging technologies that will further our understanding of transcriptional regulation.

转录因子动力学:一次一个分子。
细胞必须严格调节其基因表达程序,同时对环境中的急性生化和生物物理线索做出快速反应。这些信息通过各种信号级联传递到细胞核,最终激活或抑制靶基因。转录因子(TF)是这些信号的关键介质,与染色质内的特定调节元件结合。虽然活细胞成像已经最终证明TF-染色质相互作用是高度动态的,但这种短暂的相互作用如何对发育轨迹和疾病进展产生长期影响在很大程度上仍不清楚。在这篇综述中,我们总结了我们目前对TF功能动态性质的理解,从早期活细胞实验的历史概述开始。我们强调了控制TF动力学的关键因素,以及TF动力学如何反过来影响下游转录爆发。最后,我们总结了开放的挑战和新兴的技术,这些技术将进一步加深我们对转录调控的理解。
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来源期刊
CiteScore
19.50
自引率
0.00%
发文量
21
期刊介绍: The Annual Review of Cell and Developmental Biology, established in 1985, comprehensively addresses major advancements in cell and developmental biology. Encompassing the structure, function, and organization of cells, as well as the development and evolution of cells in relation to both single and multicellular organisms, the journal explores models and tools of molecular biology. As of the current volume, the journal has transitioned from gated to open access through Annual Reviews' Subscribe to Open program, making all articles published under a CC BY license.
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