Cytocompatibility and cell migration evaluation of calcium silicate-based root canal sealer compared to epoxide-amine resin sealer in stem cells from human apical papilla: An in-vitro study

IF 1.3 4区医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Australian Endodontic Journal Pub Date : 2023-08-03 DOI:10.1111/aej.12786

Linh Cao Hoai Nguyen DDS, MSc, Loc Nguyen Gia Pham DDS, Khoa Van Pham DDS, PhD, Minh Duc Nguyen DDS, MSc, Lan Thi Quynh Ngo DDS, PhD

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Abstract

The purpose of this study was to assess the effect of a calcium silicate-based sealers (CeraSeal) and an epoxy resin-based sealer (AH Plus) on cytotoxicity and cell migration of stem cell from the human apical papilla (hSCAPs) by using the Alamar Blue, Annexin V-FICT and wound healing assays. In Alamar Blue assay, hSCAPs exposed to undiluted CeraSeal extract had significantly higher cell viability compared with that observed when cells were treated with AH Plus in all experimental period (p < 0.001). The flow cytometry analysis confirmed the comparison on viable cells and indicated that AH Plus increased apoptosis compared to CeraSeal and the control groups (p < 0.001). Additionally, AH Plus exhibited significantly lower level of cell migration than CeraSeal and the control for up to 48 h observation (p < 0.01). In summary, calcium silicate-based sealer (CeraSeal) is less cytotoxic and more biocompatible than epoxy resin-based sealer (AH Plus).

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硅酸钙型根管封闭剂与环氧胺树脂封闭剂在人类根尖乳头干细胞中的细胞相容性和细胞迁移评估：体外研究

本研究的目的是通过阿拉玛蓝（Alamar Blue）、附录素 V-FICT 和伤口愈合试验，评估硅酸钙封孔剂（CeraSeal）和环氧树脂封孔剂（AH Plus）对人乳头顶端干细胞（hSCAPs）的细胞毒性和细胞迁移的影响。在阿拉玛蓝检测中，在所有实验期间，接触未稀释 CeraSeal 提取物的 hSCAPs 的细胞存活率都明显高于用 AH Plus 处理的细胞（p < 0.001）。流式细胞仪分析证实了对存活细胞的比较，并表明与 CeraSeal 和对照组相比，AH Plus 增加了细胞凋亡（p < 0.001）。此外，在长达 48 小时的观察中，AH Plus 的细胞迁移水平明显低于 CeraSeal 和对照组（p < 0.01）。总之，硅酸钙封闭剂（CeraSeal）比环氧树脂封闭剂（AH Plus）的细胞毒性更低，生物相容性更好。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Australian Endodontic Journal DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.50

自引率

6.20%

发文量

审稿时长

>12 weeks

期刊介绍： The Australian Endodontic Journal provides a forum for communication in the different fields that encompass endodontics for all specialists and dentists with an interest in the morphology, physiology, and pathology of the human tooth, in particular the dental pulp, root and peri-radicular tissues. The Journal features regular clinical updates, research reports and case reports from authors worldwide, and also publishes meeting abstracts, society news and historical endodontic glimpses. The Australian Endodontic Journal is a publication for dentists in general and specialist practice devoted solely to endodontics. It aims to promote communication in the different fields that encompass endodontics for those dentists who have a special interest in endodontics.