The patterns and diagnostic significance of the lack of surface immunoglobulin light chain on mature B cells in clinical samples for lymphoma workup

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Sara L. Huang, Thomas Fennell, Xia Chen, James Z. Huang
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引用次数: 2

Abstract

Background

Surface immunoglobulin (sIg) light chains are not always detected on mature B cells. This may present as a challenge for clonality determination in clinical flow cytometry.

Methods

To explore the mechanism and diagnostic significance of sIg negative mature B cells, we retrospectively studied 14 cases of sIg negative reactive B-cell lymphocytosis and 89 cases of sIg negative mature B-cell lymphomas. The expression patterns of sIg and cytoplasmic immunoglobulin (cIg) light chains were studied by flow cytometry using both monoclonal and polyclonal antibodies.

Results

These 14 cases of sIg negative reactive B-cell lymphocytosis were proven to be polytypic based on cytoplasmic light chain studies. In 89 cases of sIg negative mature B-cell lymphomas, we described four distinct patterns of abnormal light chain expression including partial or complete loss of sIg or cIg, suggesting different underlying mechanisms.

Conclusions

This study represents the first reported series of body or cystic fluids where reactive B cells do not have detectable sIg, arguing strongly against making a diagnosis of B-cell lymphoma based on lack of sIg in mature B cells. Since the lack of sIg does not always predict clonal/neoplastic mature B-cell proliferation, further cIg evaluation should be performed when sIg expression is not detected in mature B cells. The lack of both sIg and cIg in mature B cells may serve as a reliable surrogate clonality/neoplastic marker.

淋巴瘤临床检查成熟B细胞表面免疫球蛋白轻链缺失的模式及诊断意义
表面免疫球蛋白(sIg)轻链并不总是在成熟的B细胞中检测到。这可能是临床流式细胞术中克隆测定的一个挑战。方法回顾性分析14例sIg阴性反应性B细胞淋巴细胞增多症和89例sIg阴性成熟B细胞淋巴瘤的临床资料,探讨sIg阴性成熟B细胞的作用机制及诊断意义。单克隆抗体和多克隆抗体采用流式细胞术研究sIg和细胞质免疫球蛋白(cIg)轻链的表达谱。结果14例sIg阴性反应性b细胞增多症均为多型性。在89例sIg阴性成熟b细胞淋巴瘤中,我们描述了四种不同的异常轻链表达模式,包括sIg或cIg的部分或完全丧失,提示不同的潜在机制。本研究首次报道了反应性B细胞未检测到sIg的一系列体液或囊性液体,强烈反对根据成熟B细胞缺乏sIg来诊断B细胞淋巴瘤。由于缺乏sIg并不总是预测克隆/肿瘤成熟B细胞的增殖,因此当成熟B细胞中未检测到sIg表达时,应进行进一步的cigg评估。成熟B细胞缺乏sIg和cIg可作为可靠的克隆/肿瘤标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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