Sugarcane Bagasse-Derived Cellulose Nanocrystal/Polyvinyl Alcohol/Gum Tragacanth Composite Film Incorporated with Betel Leaf Extract as a Versatile Biomaterial for Wound Dressing.

IF 3 Q3 MATERIALS SCIENCE, BIOMATERIALS
International Journal of Biomaterials Pub Date : 2023-07-13 eCollection Date: 2023-01-01 DOI:10.1155/2023/9630168
Luong Ngoc Diem, Selorm Torgbo, Indranil Banerjee, Kunal Pal, Udomlak Sukatta, Prapassorn Rugthaworn, Prakit Sukyai
{"title":"Sugarcane Bagasse-Derived Cellulose Nanocrystal/Polyvinyl Alcohol/Gum Tragacanth Composite Film Incorporated with Betel Leaf Extract as a Versatile Biomaterial for Wound Dressing.","authors":"Luong Ngoc Diem, Selorm Torgbo, Indranil Banerjee, Kunal Pal, Udomlak Sukatta, Prapassorn Rugthaworn, Prakit Sukyai","doi":"10.1155/2023/9630168","DOIUrl":null,"url":null,"abstract":"<p><p>In this study, nanocomposite film was fabricated using cellulose nanocrystals (CNCs) as nanofiller in a polymer matrix of polyvinyl alcohol (PVA) and gum tragacanth (GT) via solution casting. CNCs were extracted from sugarcane bagasse using a steam explosion technique followed by acid hydrolysis. Initial analysis of CNCs by transmission electron microscopy (TEM) showed nanosized particles of 104 nm in length and 7 nm in width. Physical and chemical characteristics of neat PVA, PVA/GT, and PVA/GT/CNC films with varying concentrations of CNCs (from 2% to 10%) were analyzed by the scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectrometry, mechanical test, and swelling test. The SEM analysis showed cluster formation of CNCs in the polymer matrix at high concentration. The developed films were transparent. FTIR spectrometry analysis confirmed the chemical functional groups of the various components in the film. The presence of GT and CNCs in the polymer matrix improved the characteristics of films as evident in the prolonged stability for 7 days and increased mechanical properties. The highest elastic modulus of 1526.11 ± 31.86 MPa and tensile strength of 80.39 MPa were recorded in PVA/GT/CNC2 film. The swelling ability, however, decreased from 260% to 230%. Cytotoxicity analysis of the PVA/GT/CNC film showed that it is nontoxic to mouse fibroblast cells L929 with 95% cell viability. Films loaded with betel leaf extract exhibited excellent antibacterial activities against <i>Staphylococcus aureus</i> DMST 8840 and <i>Pseudomonas aeruginosa</i> TISTR 781 with 28.20 ± 0.84 mm and 23.60 ± 0.55 mm inhibition zones, respectively. These results demonstrate that PVA/GT/CNC loaded with the betel leaf extract could act as promising and versatile wound dressings to protect the wound surface from infection and dehydration.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":null,"pages":null},"PeriodicalIF":3.0000,"publicationDate":"2023-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10359141/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Biomaterials","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2023/9630168","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
引用次数: 0

Abstract

In this study, nanocomposite film was fabricated using cellulose nanocrystals (CNCs) as nanofiller in a polymer matrix of polyvinyl alcohol (PVA) and gum tragacanth (GT) via solution casting. CNCs were extracted from sugarcane bagasse using a steam explosion technique followed by acid hydrolysis. Initial analysis of CNCs by transmission electron microscopy (TEM) showed nanosized particles of 104 nm in length and 7 nm in width. Physical and chemical characteristics of neat PVA, PVA/GT, and PVA/GT/CNC films with varying concentrations of CNCs (from 2% to 10%) were analyzed by the scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectrometry, mechanical test, and swelling test. The SEM analysis showed cluster formation of CNCs in the polymer matrix at high concentration. The developed films were transparent. FTIR spectrometry analysis confirmed the chemical functional groups of the various components in the film. The presence of GT and CNCs in the polymer matrix improved the characteristics of films as evident in the prolonged stability for 7 days and increased mechanical properties. The highest elastic modulus of 1526.11 ± 31.86 MPa and tensile strength of 80.39 MPa were recorded in PVA/GT/CNC2 film. The swelling ability, however, decreased from 260% to 230%. Cytotoxicity analysis of the PVA/GT/CNC film showed that it is nontoxic to mouse fibroblast cells L929 with 95% cell viability. Films loaded with betel leaf extract exhibited excellent antibacterial activities against Staphylococcus aureus DMST 8840 and Pseudomonas aeruginosa TISTR 781 with 28.20 ± 0.84 mm and 23.60 ± 0.55 mm inhibition zones, respectively. These results demonstrate that PVA/GT/CNC loaded with the betel leaf extract could act as promising and versatile wound dressings to protect the wound surface from infection and dehydration.

Abstract Image

Abstract Image

Abstract Image

掺入槟榔叶提取物的甘蔗渣衍生纤维素纳米晶/聚乙烯醇/黄胶复合膜作为一种多功能伤口敷料生物材料。
本研究以纤维素纳米晶体(CNCs)为纳米填料,通过溶液浇注法在聚乙烯醇(PVA)和黄胶(GT)聚合物基质中制成了纳米复合薄膜。CNCs 采用蒸汽爆炸技术从甘蔗渣中提取,然后进行酸水解。利用透射电子显微镜(TEM)对 CNC 进行的初步分析显示,其为长度为 104 nm、宽度为 7 nm 的纳米级颗粒。通过扫描电子显微镜 (SEM)、傅立叶变换红外光谱 (FTIR)、机械测试和膨胀测试分析了纯 PVA、PVA/GT 和含有不同浓度 CNC 的 PVA/GT/CNC 薄膜(从 2% 到 10%)的物理和化学特性。扫描电子显微镜分析表明,在高浓度下,聚合物基质中的氯化萘形成了团簇。显影后的薄膜是透明的。傅立叶变换红外光谱分析证实了薄膜中各种成分的化学官能团。聚合物基质中 GT 和 CNC 的存在改善了薄膜的特性,表现在 7 天稳定性的延长和机械性能的提高。PVA/GT/CNC2 薄膜的弹性模量最高,为 1526.11 ± 31.86 兆帕,拉伸强度最高,为 80.39 兆帕。然而,溶胀能力从 260% 降至 230%。PVA/GT/CNC 薄膜的细胞毒性分析表明,它对小鼠成纤维细胞 L929 无毒,细胞存活率为 95%。槟榔叶提取物薄膜对金黄色葡萄球菌 DMST 8840 和铜绿假单胞菌 TISTR 781 具有出色的抗菌活性,抑菌区分别为 28.20 ± 0.84 mm 和 23.60 ± 0.55 mm。这些结果表明,含有槟榔叶提取物的 PVA/GT/CNC 可用作前景广阔的多功能伤口敷料,保护伤口表面免受感染和脱水。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
International Journal of Biomaterials
International Journal of Biomaterials MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
4.30
自引率
3.20%
发文量
50
审稿时长
21 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信