Prevalence of Sapovirus and Astrovirus in Pediatric Infectious Gastroenteritis Surveillance in Kobe City, Japan, 2016-2019.

IF 1.3 4区 医学 Q4 INFECTIOUS DISEASES
Takeshi Hanafusa, Kentaro Arikawa, Yoshihiko Tanimoto
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引用次数: 0

Abstract

Sapovirus (SaV) and astrovirus (AstV) are important viral causes of acute gastroenteritis. From 2016 to 2019, 172 stool samples were collected from children with gastroenteritis in Kobe, Japan for sentinel surveillance of infectious gastroenteritis. In this study, we tested 53 of the 172 stool samples that tested negative for other enteric viruses to determine the prevalence of SaV and AstV. The samples were screened for SaV and AstV using real-time polymerase chain reaction. Positive samples were genotyped by sequencing and genetic analysis of partial regions of the capsid and RNA-dependent RNA polymerase. Of the 53 samples tested, 19 (35.8%) were positive for SaV, and three (5.7%) were positive for AstV. Of the total samples, 11.0% (19/172) and 1.7% (3/172) were positive for SaV and AstV, respectively. The most frequently detected genotype of SaV was GI.1, followed by GII.3. The AstV genotypes were MAstV1.1 and MAstV1.4. This study indicates that SaV and AstV are important causes of viral gastroenteritis in children.

神户市2016-2019年儿童感染性胃肠炎病毒和星状病毒流行监测
腺病毒(SaV)和星状病毒(AstV)是引起急性胃肠炎的重要病毒。2016 - 2019年,在日本神户收集172例肠胃炎患儿粪便样本,对感染性肠胃炎进行哨点监测。在这项研究中,我们对172个粪便样本中的53个进行了其他肠道病毒阴性检测,以确定SaV和asv的流行程度。采用实时聚合酶链反应对样品进行SaV和AstV的筛选。阳性样品通过衣壳和RNA依赖性RNA聚合酶部分区域的测序和遗传分析进行基因分型。53份检测样本中,SaV阳性19份(35.8%),AstV阳性3份(5.7%)。SaV和AstV阳性率分别为11.0%(19/172)和1.7%(3/172)。最常见的SaV基因型为GI.1,其次为gi .3。AstV基因型分别为MAstV1.1和MAstV1.4。本研究提示SaV和AstV是儿童病毒性胃肠炎的重要病因。
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来源期刊
CiteScore
4.50
自引率
4.50%
发文量
172
审稿时长
2 months
期刊介绍: Japanese Journal of Infectious Diseases (JJID), an official bimonthly publication of National Institute of Infectious Diseases, Japan, publishes papers dealing with basic research on infectious diseases relevant to humans in the fields of bacteriology, virology, mycology, parasitology, medical entomology, vaccinology, and toxinology. Pathology, immunology, biochemistry, and blood safety related to microbial pathogens are among the fields covered. Sections include: original papers, short communications, epidemiological reports, methods, laboratory and epidemiology communications, letters to the editor, and reviews.
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