Development of an in vitro Functional Assay to Evaluate the Occlusive Properties of Moisturizers on Dry Skin.

IF 2.8 4区医学 Q2 DERMATOLOGY

Skin Pharmacology and Physiology Pub Date : 2023-01-01 DOI:10.1159/000529630

Soonjin Hong, Prithwiraj Maitra, Audrey Nguyen, Kuniko Kadoya, Rahul C Mehta, Elizabeth SelwanLewis

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引用次数: 1

Abstract

Introduction: Dry skin is a hallmark of impaired skin barrier function. Moisturizers are a mainstay of treatment to help the skin retain moisture, and there is a high consumer demand for effective products. However, the development and optimization of new formulations are hampered due to lack of reliable efficacy measures using in vitro models.

Methods: In this study, a microscopy-based barrier functional assay was developed using an in vitro skin model of chemically induced barrier damage to evaluate the occlusive activity of moisturizers.

Results: The assay was validated by demonstrating the different effects on barrier function between humectant (glycerol) and occlusive (petrolatum). Significant changes in barrier function were observed upon tissue disruption, which was ameliorated by commercial moisturizing products.

Conclusion: This newly developed experimental method may be helpful to develop new and improved occlusive moisturizers for the treatment of dry skin conditions.

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开发一种体外功能试验来评估保湿剂对干燥皮肤的封闭特性。

皮肤干燥是皮肤屏障功能受损的标志。保湿霜是帮助皮肤保持水分的主要治疗方法，消费者对有效产品的需求很高。然而，由于缺乏可靠的体外模型疗效测量，新配方的开发和优化受到阻碍。方法:在本研究中，利用化学诱导屏障损伤的体外皮肤模型，建立了一种基于显微镜的屏障功能测定方法，以评估润肤霜的闭塞活性。结果:湿剂(甘油)和闭塞剂(凡士林)对屏障功能的影响不同，验证了该方法的有效性。在组织破坏时观察到屏障功能的显著变化，这是通过商业保湿产品改善的。结论:本实验方法有助于开发新型和改良的闭塞性保湿剂治疗皮肤干燥。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Skin Pharmacology and Physiology 医学-皮肤病学

CiteScore

5.20

自引率

7.40%

发文量

审稿时长

>12 weeks

期刊介绍： In the past decade research into skin pharmacology has rapidly developed with new and promising drugs and therapeutic concepts being introduced regularly. Recently, the use of nanoparticles for drug delivery in dermatology and cosmetology has become a topic of intensive research, yielding remarkable and in part surprising results. Another topic of current research is the use of tissue tolerable plasma in wound treatment. Stimulating not only wound healing processes but also the penetration of topically applied substances into the skin, this novel technique is expected to deliver very interesting results.