A pre-clinical study to investigate the anti-diabetic potential of p-propoxybenzoic acid as a multi-target inhibitor in streptozotocin-nicotinamide induced type-2 diabetic rats.
{"title":"A pre-clinical study to investigate the anti-diabetic potential of <i>p</i>-propoxybenzoic acid as a multi-target inhibitor in streptozotocin-nicotinamide induced type-2 diabetic rats.","authors":"Keval Y Raval, Pravin R Tirgar","doi":"10.1007/s40200-022-01177-y","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>The study was undertaken to evaluate the anti-diabetic potential of <i>p-</i>propoxybenzoic acid (<i>p-</i>PBA).</p><p><strong>Methods: </strong>36 Sprague-Dawley rats of either sex were utilized for the study. Animals were injected with nicotinamide (230 mg/kg) followed by streptozotocin (65 mg/kg) to induce Type-2 Diabetes (T2DM). Animals with blood glucose levels (BGL) over 200 mg/kg were allocated in six groups. Three groups were treated with <i>p</i>-PBA dose of 100 mg/kg, 200 mg/kg and 300 mg/kg respectively; standard control group was treated with 5 mg/kg glibenclamide, while the other two groups were considered as normal control and disease control group. Body weight (BW) and BGL were recorded on Day 0, Day 7, Day 14, and Day 28. Glycosylated hemoglobin (HbA1c), serum insulin levels and lipid profile were recorded on Day 28. Animals were euthanized on Day 28 and the pancreas was isolated for histopathological examination.</p><p><strong>Results: </strong>Diabetic animals treated with <i>p-</i>PBA showed significant improvements in BW (<i>P</i> < 0.05) and BGL (<i>P</i> < 0.001) over 28 days. Levels of HbA1c (<i>P</i> < 0.05) and serum insulin (<i>P</i> < 0.001) were significantly regulated in animals treated with <i>p-</i>PBA. A significant decrease (<i>P</i> < 0.001) was observed in elevated levels of TC, TG, LDL cholesterol and VLDL cholesterol in animals treated with <i>p-</i>PBA. <i>p</i>-PBA significantly regulated the levels of HDL cholesterol (<i>P</i> < 0.001). A notable protective effect of <i>p-</i>PBA was observed through the histopathological examination of pancreas.</p><p><strong>Conclusion: </strong><i>p-</i>PBA can be characterized as a multi-target inhibiting anti-diabetic agent which can be evaluated against diabetic complications.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s40200-022-01177-y.</p>","PeriodicalId":15635,"journal":{"name":"Journal of Diabetes and Metabolic Disorders","volume":"22 1","pages":"571-580"},"PeriodicalIF":1.8000,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10225424/pdf/","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Diabetes and Metabolic Disorders","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/s40200-022-01177-y","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/6/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"ENDOCRINOLOGY & METABOLISM","Score":null,"Total":0}
引用次数: 3
Abstract
Purpose: The study was undertaken to evaluate the anti-diabetic potential of p-propoxybenzoic acid (p-PBA).
Methods: 36 Sprague-Dawley rats of either sex were utilized for the study. Animals were injected with nicotinamide (230 mg/kg) followed by streptozotocin (65 mg/kg) to induce Type-2 Diabetes (T2DM). Animals with blood glucose levels (BGL) over 200 mg/kg were allocated in six groups. Three groups were treated with p-PBA dose of 100 mg/kg, 200 mg/kg and 300 mg/kg respectively; standard control group was treated with 5 mg/kg glibenclamide, while the other two groups were considered as normal control and disease control group. Body weight (BW) and BGL were recorded on Day 0, Day 7, Day 14, and Day 28. Glycosylated hemoglobin (HbA1c), serum insulin levels and lipid profile were recorded on Day 28. Animals were euthanized on Day 28 and the pancreas was isolated for histopathological examination.
Results: Diabetic animals treated with p-PBA showed significant improvements in BW (P < 0.05) and BGL (P < 0.001) over 28 days. Levels of HbA1c (P < 0.05) and serum insulin (P < 0.001) were significantly regulated in animals treated with p-PBA. A significant decrease (P < 0.001) was observed in elevated levels of TC, TG, LDL cholesterol and VLDL cholesterol in animals treated with p-PBA. p-PBA significantly regulated the levels of HDL cholesterol (P < 0.001). A notable protective effect of p-PBA was observed through the histopathological examination of pancreas.
Conclusion: p-PBA can be characterized as a multi-target inhibiting anti-diabetic agent which can be evaluated against diabetic complications.
Supplementary information: The online version contains supplementary material available at 10.1007/s40200-022-01177-y.
目的：评价对丙氧基苯甲酸（p-PBA）的抗糖尿病作用。动物注射烟酰胺（230 mg/kg），然后注射链脲佐菌素（65 mg/kg）以诱导2型糖尿病（T2DM）。将血糖水平（BGL）超过200mg/kg的动物分为六组。三组分别用100mg/kg、200mg/kg和300mg/kg的p-PBA剂量进行治疗；标准对照组给予格列本脲5mg/kg，其余两组分别为正常对照组和疾病对照组。在第0天、第7天、第14天和第28天记录体重（BW）和BGL。在第28天记录糖化血红蛋白（HbA1c）、血清胰岛素水平和脂质状况。在第28天对动物实施安乐死，并分离胰腺进行组织病理学检查。结果：p-PBA对糖尿病动物的BW有显著改善（p P P P p-PBA。显著下降（P p-PBA。p-PBA显著调节HDL胆固醇水平（p 胰腺组织病理学检查观察p-PBA。结论：p-PBA是一种多靶点的抗糖尿病药物，可用于糖尿病并发症的评估。补充信息：在线版本包含补充材料，请访问10.1007/s40200-022-01177-y。
期刊介绍：
Journal of Diabetes & Metabolic Disorders is a peer reviewed journal which publishes original clinical and translational articles and reviews in the field of endocrinology and provides a forum of debate of the highest quality on these issues. Topics of interest include, but are not limited to, diabetes, lipid disorders, metabolic disorders, osteoporosis, interdisciplinary practices in endocrinology, cardiovascular and metabolic risk, aging research, obesity, traditional medicine, pychosomatic research, behavioral medicine, ethics and evidence-based practices.As of Jan 2018 the journal is published by Springer as a hybrid journal with no article processing charges. All articles published before 2018 are available free of charge on springerlink.Unofficial 2017 2-year Impact Factor: 1.816.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
