Comparative expression analysis of TEADs and their splice variants in mouse embryonic stem cells

IF 1 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

Gene Expression Patterns Pub Date : 2023-03-01 DOI:10.1016/j.gep.2022.119302

Yuda Cheng , Yang Xiao , Yan Ruan , Jiali Wang , Yanping Tian , Jiaxiang Xiong , Jiaqi Wang , Fengsheng Wang , Chen Zhang , Yixiao Xu , Lianlian Liu , Meng Yu , Jiangjun Wang , Binyu Zhao , Yue Zhang , Ran Yang , Yi Yang , Zhongxiang Yao , Rui Jian , Lan Xiao , Junlei Zhang

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引用次数: 0

Abstract

Transcriptional enhanced associate domain (TEAD) transcription factors play important roles in embryonic stem cell (ESC) renewal and differentiation. Four TEAD transcription factors (Tead1, Tead2, Tead3 and Tead4) and their various splice variants have been discovered in mice, but the expression pattern of them during pluripotency state transition is unclear. Here, we investigated the expression of TEADs and their splice variants in mouse ESCs at different pluripotent/differentiating states and adult mouse tissues. Our results preliminarily revealed the diversity and heterogeneity of TEAD family, which is helpful for understanding their overlapping and distinctive functions. Furthermore, a novel splice variant of Tead1 was identified and named Tead1 isoform 4.

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TEADs及其剪接变异体在小鼠胚胎干细胞中的比较表达分析

转录增强相关结构域(TEAD)转录因子在胚胎干细胞(ESC)的更新和分化中起着重要作用。在小鼠中已经发现了四种TEAD转录因子(Tead1、Tead2、Tead3和Tead4)及其各种剪接变异体，但它们在多能状态转变过程中的表达模式尚不清楚。在这里，我们研究了TEADs及其剪接变体在不同多能/分化状态的小鼠ESCs和成年小鼠组织中的表达。我们的研究结果初步揭示了TEAD家族的多样性和异质性，有助于了解它们的重叠性和独特功能。此外，还鉴定出一种新的Tead1剪接变体，并将其命名为Tead1亚型4。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gene Expression Patterns 生物-发育生物学

CiteScore

2.30

自引率

0.00%

发文量

审稿时长

35 days

期刊介绍： Gene Expression Patterns is devoted to the rapid publication of high quality studies of gene expression in development. Studies using cell culture are also suitable if clearly relevant to development, e.g., analysis of key regulatory genes or of gene sets in the maintenance or differentiation of stem cells. Key areas of interest include: -In-situ studies such as expression patterns of important or interesting genes at all levels, including transcription and protein expression -Temporal studies of large gene sets during development -Transgenic studies to study cell lineage in tissue formation