A tri-layer decellularized, dehydrated human amniotic membrane scaffold supports the cellular functions of human tenocytes in vitro

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine Pub Date : 2023-07-24 DOI:10.1007/s10856-023-06740-4

Yong Mao, Nikita John, Nicole M. Protzman, Desiree Long, Raja Sivalenka, Shamshad Azimi, Brandon Mirabile, Robert Pouliot, Anna Gosiewska, Robert J. Hariri, Stephen A. Brigido

引用次数: 0

Abstract

Differences in scaffold design have the potential to influence cell-scaffold interactions. This study sought to determine whether a tri-layer design influences the cellular function of human tenocytes in vitro. The single-layer decellularized, dehydrated human amniotic membrane (DDHAM) and the tri-layer DDHAM (DDHAM-3L) similarly supported tenocyte function as evidenced by improved cell growth and migration, reduced dedifferentiation, and an attenuated inflammatory response. The tri-layer design provides a mechanically more robust scaffold without altering biological activity.

Graphical Abstract

Abstract Image

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三层脱细胞脱水人羊膜支架支持体外人腱鞘细胞的细胞功能

支架设计的不同有可能影响细胞与支架之间的相互作用。本研究试图确定三层设计是否会影响体外人类腱细胞的细胞功能。单层脱细胞脱水人羊膜（DDHAM）和三层脱细胞脱水人羊膜（DDHAM-3L）同样支持腱细胞功能，这表现在细胞生长和迁移得到改善、去分化减少以及炎症反应减弱。三层设计在不改变生物活性的情况下提供了一种机械上更坚固的支架。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Materials Science: Materials in Medicine 工程技术-材料科学：生物材料

CiteScore

8.00

自引率

0.00%

发文量

审稿时长

3.5 months

期刊介绍： The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.