lncRNA EGFEM1P Drives the Progression of Papillary Thyroid Cancer by Regulating miR-6867-5p/CHI3L1 Axis.

IF 1.5 4区 医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Zhanwu Ma, Guoxian Wang, Lin Hu
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引用次数: 0

Abstract

Long noncoding RNA (lncRNA), a subgroup of noncoding RNA with > 200 nt, plays critical roles in cancer progression. Here, we aimed to explore the detailed biological function of lncRNA EGFEM1P during papillary thyroid cancer (PTC) progression. RT-qPCR and Western blot were used to analyze the expression of lncRNA EGFEM1P, miR-6867-5p, and CHI3L1. CCK8, colony formation, and Transwell migration assays were undertaken to assess PTC cell proliferation and migration. A xenograft tumor mouse model was also used to establish tumor growth in vivo. Luciferase reporter and anti-AGO2 RNA immunoprecipitation (RIP) assays were used to clarify the interplay between miR-6867-5p and lncRNA EGFEM1P or CHI3L1. We found lncRNA EGFEM1P and CHI3L1 to be highly expressed in PTC tissues and cells, while miR-6867-5p expression decreases. Functionally, lncRNA EGFEM1P silence delays PTC cell proliferation and migration, and impairs tumorigenesis in vivo. LncRNA EGFEM1P targets miR-6867-5p, and CHI3L1 is a target gene of miR-6867-5p. LncRNA EGFEM1P silence decreases the pro-proliferation and pro-migration caused by the miR-6867-5p inhibitor in PTC cells, and CHI3L1 silence abrogates the pro-tumorigenic action resulting from the miR-6867-5p inhibitor in PTC cells. Our data showed that lncRNA EGFEM1P targeting of the miR-6867-5p/CHI3L1 axis drives PTC progression, suggesting lncRNA EGFEM1P as a therapeutically target for PTC.

lncRNA EGFEM1P通过调节miR-6867-5p/CHI3L1轴驱动甲状腺乳头状癌的进展
长链非编码RNA (lncRNA)是长度大于200 nt的非编码RNA亚群,在癌症进展中起着关键作用。在这里,我们旨在探讨lncRNA EGFEM1P在乳头状甲状腺癌(PTC)进展中的详细生物学功能。采用RT-qPCR和Western blot分析lncRNA EGFEM1P、miR-6867-5p、CHI3L1的表达。CCK8、菌落形成和Transwell迁移试验评估PTC细胞的增殖和迁移。异种移植瘤小鼠模型也被用来建立肿瘤在体内的生长。使用荧光素酶报告基因和抗ago2 RNA免疫沉淀(RIP)测定来阐明miR-6867-5p与lncRNA EGFEM1P或CHI3L1之间的相互作用。我们发现lncRNA EGFEM1P和CHI3L1在PTC组织和细胞中高表达,miR-6867-5p表达降低。在功能上,lncRNA EGFEM1P沉默延迟PTC细胞的增殖和迁移,并在体内损害肿瘤发生。LncRNA EGFEM1P靶向miR-6867-5p, CHI3L1是miR-6867-5p的靶基因。LncRNA EGFEM1P沉默降低了miR-6867-5p抑制剂在PTC细胞中的促增殖和促迁移作用,CHI3L1沉默消除了miR-6867-5p抑制剂在PTC细胞中的促肿瘤作用。我们的数据显示,lncRNA EGFEM1P靶向miR-6867-5p/CHI3L1轴驱动PTC进展,这表明lncRNA EGFEM1P是PTC的治疗靶点。
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来源期刊
Critical Reviews in Eukaryotic Gene Expression
Critical Reviews in Eukaryotic Gene Expression 生物-生物工程与应用微生物
CiteScore
2.70
自引率
0.00%
发文量
67
审稿时长
1 months
期刊介绍: Critical ReviewsTM in Eukaryotic Gene Expression presents timely concepts and experimental approaches that are contributing to rapid advances in our mechanistic understanding of gene regulation, organization, and structure within the contexts of biological control and the diagnosis/treatment of disease. The journal provides in-depth critical reviews, on well-defined topics of immediate interest, written by recognized specialists in the field. Extensive literature citations provide a comprehensive information resource. Reviews are developed from an historical perspective and suggest directions that can be anticipated. Strengths as well as limitations of methodologies and experimental strategies are considered.
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