Critical Evaluation of Transcripts and Long Noncoding RNA Expression Levels in Prostate Cancer Following Radical Prostatectomy.

IF 3.5 4区 医学 Q3 CELL BIOLOGY
Pathobiology Pub Date : 2023-01-01 Epub Date: 2023-07-18 DOI:10.1159/000531175
Christian Ruiz, Ilaria Alborelli, Massimilinao Manzo, Byron Calgua, Eveline Barbara Keller, Vincent Vuaroqueaux, Luca Quagliata, Cyrill A Rentsch, Giulio Cesare Spagnoli, Pierre André Diener, Lukas Bubendorf, Rudolf Morant, Serenella Eppenberger-Castori
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引用次数: 0

Abstract

Introduction: The clinical course of prostate cancer (PCa) is highly variable, ranging from indolent behavior to rapid metastatic progression. The Gleason score is widely accepted as the primary histologic assessment tool with significant prognostic value. However, additional biomarkers are required to better stratify patients, particularly those at intermediate risk.

Methods: In this study, we analyzed the expression of 86 cancer hallmark genes in 171 patients with PCa who underwent radical prostatectomy and focused on the outcome of the 137 patients with postoperative R0-PSA0 status.

Results: Low expression of the IGF1 and SRD52A, and high expression of TIMP2, PLAUR, S100A2, and CANX genes were associated with biochemical recurrence (BR), defined as an increase of prostate-specific antigen above 0.2 ng/mL. Furthermore, the analysis of the expression of 462 noncoding RNAs (ncRNA) in a sub-cohort of 39 patients with Gleason score 7 tumors revealed that high levels of expression of the ncRNAs LINC00624, LINC00593, LINC00482, and cd27-AS1 were significantly associated with BR. Our findings provide further evidence for tumor-promoting roles of ncRNAs in PCa patients at intermediate risk. The strong correlation between expression of LINC00624 and KRT8 gene, encoding a well-known cell surface protein present in PCa, further supports a potential contribution of this ncRNA to PCa progression.

Conclusion: While larger and further studies are needed to define the role of these genes/ncRNA in PCa, our findings pave the way toward the identification of a subgroup of patients at intermediate risk who may benefit from adjuvant treatments and new therapeutic agents.

前列腺癌根治术后转录本和长非编码 RNA 表达水平的关键评估。
简介前列腺癌(PCa)的临床病程变化很大,从症状不明显到迅速转移。格里森评分是公认的主要组织学评估工具,具有重要的预后价值。然而,还需要更多的生物标志物来更好地对患者进行分层,尤其是中危患者:在这项研究中,我们分析了接受根治性前列腺切除术的 171 例 PCa 患者中 86 个癌症标志基因的表达情况,并重点研究了 137 例术后 R0-PSA0 状态患者的预后:结果:IGF1和SRD52A基因的低表达以及TIMP2、PLAUR、S100A2和CANX基因的高表达与生化复发(BR)有关,生化复发的定义是前列腺特异性抗原升高超过0.2纳克/毫升。此外,对 39 例格里森评分 7 分肿瘤患者的子队列中 462 个非编码 RNA(ncRNA)的表达进行分析后发现,ncRNA LINC00624、LINC00593、LINC00482 和 cd27-AS1 的高水平表达与生化复发显著相关。我们的研究结果进一步证明了 ncRNA 在中危 PCa 患者中的肿瘤促进作用。LINC00624的表达与KRT8基因之间的强相关性进一步证实了该ncRNA对PCa进展的潜在作用:我们的研究结果为确定中危患者亚群铺平了道路,这些患者可能会从辅助治疗和新的治疗药物中获益。
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来源期刊
Pathobiology
Pathobiology 医学-病理学
CiteScore
8.50
自引率
0.00%
发文量
47
审稿时长
>12 weeks
期刊介绍: ''Pathobiology'' offers a valuable platform for the publication of high-quality original research into the mechanisms underlying human disease. Aiming to serve as a bridge between basic biomedical research and clinical medicine, the journal welcomes articles from scientific areas such as pathology, oncology, anatomy, virology, internal medicine, surgery, cell and molecular biology, and immunology. Published bimonthly, ''Pathobiology'' features original research papers and reviews on translational research. The journal offers the possibility to publish proceedings of meetings dedicated to one particular topic.
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