Molecular evolution steered structural adaptations in the DNA polymerase III α subunit of halophilic bacterium Salinibacter ruber.

IF 2.6 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Aveepsa Sengupta, Kunwali Das, Nidhi Jha, Yusuf Akhter, Ashutosh Kumar
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Abstract

A significant portion of the earth has a salty environment, and the literature on bacterial survival mechanisms in salty environments is limited. During molecular evolution, halophiles increase acidic amino acid residues on their protein surfaces which leads to a negatively charged surface potential that helps them to maintain the protein integrity and protect them from denaturation by competing with salt ions. Through protein family analysis, we have investigated the molecular-level adaptive features of DNA polymerase III's catalytic subunit (alpha) and its structure-function relationship. This study throws light on the novel understanding of halophilic bacterial replication and the molecular basis of salt adaptation. Comparisons of the amino acid contents and electronegativity of halophilic and mesophilic bacterial proteins revealed adaptations that allow halophilic bacteria to thrive in high salt concentrations. A significantly lower isoelectric point of halophilic bacterial proteins indicates the acidic nature. Also, an abundance of disordered regions in halophiles suggests the requirement of the salt ions that play a crucial role in their stable protein folding. Despite having similar topology, mesophilic and halophilic proteins, a set of very prominent molecular modifications was observed in the alpha subunit of halophiles.

Abstract Image

嗜盐细菌橡胶盐杆菌DNA聚合酶III α亚基的分子进化引导结构适应。
地球上有很大一部分是咸水环境,关于细菌在咸水环境中生存机制的文献有限。在分子进化过程中,嗜盐菌在其蛋白质表面增加酸性氨基酸残基,从而导致带负电荷的表面电位,这有助于它们保持蛋白质的完整性,并保护它们不因与盐离子竞争而变性。通过蛋白家族分析,我们研究了DNA聚合酶III催化亚基(α)的分子水平适应特征及其结构-功能关系。该研究对嗜盐细菌的复制和盐适应的分子基础有了新的认识。对嗜盐细菌和中温细菌蛋白质的氨基酸含量和电负性的比较揭示了嗜盐细菌在高盐浓度下茁壮成长的适应性。嗜盐细菌蛋白质的等电点明显较低表明其酸性。此外,嗜盐菌中大量的无序区域表明对盐离子的需求在其稳定的蛋白质折叠中起着至关重要的作用。尽管具有相似的拓扑结构,嗜中温和亲盐蛋白,但在嗜盐菌的α亚基中观察到一组非常突出的分子修饰。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Extremophiles
Extremophiles 生物-生化与分子生物学
CiteScore
6.80
自引率
6.90%
发文量
28
审稿时长
2 months
期刊介绍: Extremophiles features original research articles, reviews, and method papers on the biology, molecular biology, structure, function, and applications of microbial life at high or low temperature, pressure, acidity, alkalinity, salinity, or desiccation; or in the presence of organic solvents, heavy metals, normally toxic substances, or radiation.
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