The Battle Against Quenching and Fading for Fluorescence: A Model of How to Evaluate the Fluorophore's Behavior.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-07-01 Epub Date: 2023-07-07 DOI:10.1369/00221554231185184

Gloria E Hoffman

引用次数: 0

Abstract

The fading and quenching of fluorescence intensity has been a major problem in the use of fluorescein isothiocyanate (FITC) for immunofluorescence cytochemical techniques, especially with laser confocal microscopy. The companion article by Longin et al. provided an empirical approach to overcoming this problem. The present commentary highlights the significance of the Longin et al. article when it was published and its continued relevance today.

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对抗荧光的淬灭和褪色：如何评估荧光团行为的模型。

荧光强度的衰减和淬灭一直是异硫氰酸荧光素（FITC）用于免疫荧光细胞化学技术，特别是激光共聚焦显微镜时的一个主要问题。Longin 等人的相关文章提供了克服这一问题的经验方法。本评论强调了 Longin 等人的文章在发表时的重要意义及其在今天的现实意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.