The Battle Against Quenching and Fading for Fluorescence: A Model of How to Evaluate the Fluorophore's Behavior.

IF 1.9 4区生物学 Q4 CELL BIOLOGY

Journal of Histochemistry & Cytochemistry Pub Date : 2023-07-01 Epub Date: 2023-07-07 DOI:10.1369/00221554231185184

Gloria E Hoffman

引用次数: 0

Abstract

The fading and quenching of fluorescence intensity has been a major problem in the use of fluorescein isothiocyanate (FITC) for immunofluorescence cytochemical techniques, especially with laser confocal microscopy. The companion article by Longin et al. provided an empirical approach to overcoming this problem. The present commentary highlights the significance of the Longin et al. article when it was published and its continued relevance today.

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对抗荧光的淬灭和褪色：如何评估荧光团行为的模型。

荧光强度的衰减和淬灭一直是异硫氰酸荧光素（FITC）用于免疫荧光细胞化学技术，特别是激光共聚焦显微镜时的一个主要问题。Longin 等人的相关文章提供了克服这一问题的经验方法。本评论强调了 Longin 等人的文章在发表时的重要意义及其在今天的现实意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Histochemistry & Cytochemistry 生物-细胞生物学

CiteScore

6.00

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Journal of Histochemistry & Cytochemistry (JHC) has been a pre-eminent cell biology journal for over 50 years. Published monthly, JHC offers primary research articles, timely reviews, editorials, and perspectives on the structure and function of cells, tissues, and organs, as well as mechanisms of development, differentiation, and disease. JHC also publishes new developments in microscopy and imaging, especially where imaging techniques complement current genetic, molecular and biochemical investigations of cell and tissue function. JHC offers generous space for articles and recognizing the value of images that reveal molecular, cellular and tissue organization, offers free color to all authors.