Static Magnetic Field Promotes Proliferation, Migration, Differentiation, and AKT Activation of Periodontal Ligament Stem Cells.

IF 2.9 4区 生物学 Q1 ANATOMY & MORPHOLOGY
Cells Tissues Organs Pub Date : 2023-01-01 Epub Date: 2022-03-28 DOI:10.1159/000524291
Kun Zhang, WenBin Ge, ShiTong Luo, Zhi Zhou, YaLi Liu
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引用次数: 3

Abstract

Periodontal ligament stem cells (PDLSCs) possess self-renewal and multilineage differentiation potential and exhibit great potential for the treatment of bone tissue defects caused by inflammation. Previous studies have indicated that static magnetic field (SMF) can enhance the proliferation and differentiation of mesenchymal stem cells (MSCs). SMF has been widely used to repair bone defects and for orthodontic and implantation treatment. In this study, we revealed that a 320 mT SMF upregulates the protein expression levels of cytokines such as MCM7 and PCNA in proliferating PDLSCs. Cell counting kit-8 results revealed that the SMF group had higher optical density values than the control group. The ratio of cells in the S phase to those in the G2/M phase was significantly increased after exposure to a 320 mT SMF. In scratch assays, the SMF-treated PDLSCs exhibited a higher migration rate than the sham-exposed group after 24 h of culture, indicating that the SMF promoted the migratory ability of PDLSCs. The activity level of the early differentiation marker alkaline phosphatase and the late marker matrix mineralization, as well as osteoblast-specific gene and protein expression, were enhanced in PDLSCs exposed to the SMF. Furthermore, AKT signaling pathway was activated by SMF. Our data demonstrated that the potential mechanism of action of SMF may enhance PDLSCs proliferation and osteogenic differentiation by activating the phosphorylated AKT pathway. The elucidation of this molecular mechanism may lead to a better understanding of bone repair responses and aid in improved stem cell-mediated regeneration.

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静态磁场促进牙周韧带干细胞的增殖、迁移、分化和AKT激活。
牙周韧带干细胞(PDLSCs)具有自我更新和多谱系分化的潜力,在治疗炎症引起的骨组织缺损方面表现出巨大潜力。先前的研究表明,静磁场(SMF)可以促进间充质干细胞(MSC)的增殖和分化。SMF已被广泛用于修复骨缺损以及用于正畸和植入治疗。在这项研究中,我们发现320mT SMF上调了增殖的PDLSCs中细胞因子如MCM7和PCNA的蛋白表达水平。细胞计数试剂盒-8的结果显示,SMF组的光密度值高于对照组。暴露于320mT SMF后,S期细胞与G2/M期细胞的比例显著增加。在划痕试验中,SMF处理的PDLSCs在培养24小时后表现出比假暴露组更高的迁移率,表明SMF促进了PDLSCs的迁移能力。暴露于SMF的PDLSCs中,早期分化标志物碱性磷酸酶和晚期标志物基质矿化的活性水平以及成骨细胞特异性基因和蛋白质表达增强。此外,AKT信号通路被SMF激活。我们的数据表明,SMF的潜在作用机制可能通过激活磷酸化的AKT途径来增强PDLSCs的增殖和成骨分化。阐明这种分子机制可以更好地理解骨修复反应,并有助于改善干细胞介导的再生。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Cells Tissues Organs
Cells Tissues Organs 生物-发育生物学
CiteScore
4.90
自引率
3.70%
发文量
45
审稿时长
6-12 weeks
期刊介绍: ''Cells Tissues Organs'' aims at bridging the gap between cell biology and developmental biology and the emerging fields of regenerative medicine (stem cell biology, tissue engineering, artificial organs, in vitro systems and transplantation biology). CTO offers a rapid and fair peer-review and exquisite reproduction quality. Special topic issues, entire issues of the journal devoted to a single research topic within the range of interests of the journal, are published at irregular intervals.
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