The abundance of the potential pathogen Staphylococcus hominis in the air microbiome in a dental clinic and its susceptibility to far-UVC light

IF 3.9 3区生物学 Q2 MICROBIOLOGY

MicrobiologyOpen Pub Date : 2023-03-05 DOI:10.1002/mbo3.1348

Marilena Aquino de Muro, Igor Shuryak, Anne-Catrin Uhlemann, Alice Tillman, Dwayne Seeram, Joseph Zakaria, David Welch, Steven M. Erde, David J. Brenner

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Abstract

The dental clinic air microbiome incorporates microbes from the oral cavity and upper respiratory tract (URT). This study aimed to establish a reliable methodology for air sampling in a dental clinic setting and quantify the abundance of culturable mesophilic aerobic bacteria present in these samples using regression modeling. Staphylococcus hominis, a potentially pathogenic bacterium typically found in the human oropharynx and URT, was consistently isolated. S. hominis was the most abundant species of aerobic bacteria (22%–24%) and comprised 60%–80% of all Staphylococcus spp. The study also assessed the susceptibility of S. hominis to 222 nm-far-UVC light in laboratory experiments, which showed an exponential surface inactivation constant of k = 0.475 cm²/mJ. This constant is a critical parameter for future on-site use of far-UVC light as a technique for reducing pathogenic bacterial load in dental clinics.

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牙科诊所空气微生物群中潜在病原体人型葡萄球菌的丰度及其对远紫外线光的敏感性

牙科诊所空气微生物组包括来自口腔和上呼吸道(URT)的微生物。本研究旨在建立一种可靠的口腔诊所空气采样方法，并使用回归模型量化这些样本中存在的可培养的中温好氧细菌的丰度。人葡萄球菌是一种潜在的致病性细菌，通常在人类口咽和上呼吸道发现，一直被分离出来。人球菌是葡萄球菌中数量最多的好氧菌(22% ~ 24%)，占所有葡萄球菌的60% ~ 80%。本研究还通过室内实验评估了人球菌对222 nm远uvc光的敏感性，其指数表面失活常数k = 0.475 cm2/mJ。该常数是未来在牙科诊所现场使用远紫外线光作为减少致病菌负荷技术的关键参数。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

MicrobiologyOpen MICROBIOLOGY-

CiteScore

8.00

自引率

0.00%

发文量

审稿时长

20 weeks

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