{"title":"Transcriptome analysis reveals the role of Zelda in the regulation of embryonic and wing development of <i>Tribolium castaneum</i>.","authors":"Shanshan Gao, Shuang Xue, Tian Gao, Ruixue Lu, Xinyi Zhang, Yonglei Zhang, Kunpeng Zhang, Ruimin Li","doi":"10.1017/S0007485323000263","DOIUrl":null,"url":null,"abstract":"<p><p>Zinc finger protein (Zelda) of <i>Tribolium castaneum</i> (<i>TcZelda</i>) has been showed to play pivotal roles in embryonic development and metamorphosis. However, the regulatory mechanism of <i>TcZelda</i> associated with these physiology processes is unclear. Herein, the developmental expression profile showed that <i>Zelda</i> of <i>T. castaneum</i> was highly expressed in early eggs. Tissue expression profiling revealed that <i>TcZelda</i> was mainly expressed in the larval head and adult ovary of late adults and late larvae. <i>TcZelda</i> knockdown led to a 95% mortality rate in adults. These results suggested that <i>TcZelda</i> is related to the activation of the zygote genome in early embryonic development. Furthermore, 592 differentially expressed genes were identified from the ds<i>Zelda</i> treated group. Compared with the control group, <i>altered disjunction</i> (<i>ALD</i>) and <i>AGAP005368-PA</i> (<i>GAP</i>) in the ds<i>Zelda</i> group were significantly down-regulated, while <i>TGF-beta, propeptide</i> (<i>TGF</i>) was significantly up-regulated, suggesting that <i>TcZelda</i> may be involved in insect embryonic development. In addition, the expression of <i>Ubx ultrabithorax</i> (<i>UBX</i>), <i>Cx cephalothorax</i> (<i>CX</i>), <i>En engrailed</i> (<i>EN</i>), and two <i>Endocuticle structural glycoprotein sgabd</i> (<i>ABD</i>) genes were significantly down-regulated, suggesting that they may cooperate with <i>TcZelda</i> to regulate the development of insect wings. Additionally, <i>Elongation</i> (<i>ELO</i>), <i>fatty acid synthase</i> (<i>FAS</i>), and <i>fatty acyl-CoA desaturase</i> (<i>FAD</i>) expression was inhibited in ds<i>Zelda</i> insects, which could disturb the lipase signaling pathways, thus, disrupting the insect reproductive system and pheromone synthesis. These results may help reveal the function of <i>TcZelda</i> in insects and the role of certain genes in the gene regulatory network and provide new ideas for the prevention and control of <i>T. castaneum</i>.</p>","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":null,"pages":null},"PeriodicalIF":1.6000,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of Entomological Research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1017/S0007485323000263","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/7/21 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Zinc finger protein (Zelda) of Tribolium castaneum (TcZelda) has been showed to play pivotal roles in embryonic development and metamorphosis. However, the regulatory mechanism of TcZelda associated with these physiology processes is unclear. Herein, the developmental expression profile showed that Zelda of T. castaneum was highly expressed in early eggs. Tissue expression profiling revealed that TcZelda was mainly expressed in the larval head and adult ovary of late adults and late larvae. TcZelda knockdown led to a 95% mortality rate in adults. These results suggested that TcZelda is related to the activation of the zygote genome in early embryonic development. Furthermore, 592 differentially expressed genes were identified from the dsZelda treated group. Compared with the control group, altered disjunction (ALD) and AGAP005368-PA (GAP) in the dsZelda group were significantly down-regulated, while TGF-beta, propeptide (TGF) was significantly up-regulated, suggesting that TcZelda may be involved in insect embryonic development. In addition, the expression of Ubx ultrabithorax (UBX), Cx cephalothorax (CX), En engrailed (EN), and two Endocuticle structural glycoprotein sgabd (ABD) genes were significantly down-regulated, suggesting that they may cooperate with TcZelda to regulate the development of insect wings. Additionally, Elongation (ELO), fatty acid synthase (FAS), and fatty acyl-CoA desaturase (FAD) expression was inhibited in dsZelda insects, which could disturb the lipase signaling pathways, thus, disrupting the insect reproductive system and pheromone synthesis. These results may help reveal the function of TcZelda in insects and the role of certain genes in the gene regulatory network and provide new ideas for the prevention and control of T. castaneum.
期刊介绍：
Established in 1910, the internationally recognised Bulletin of Entomological Research aims to further global knowledge of entomology through the generalisation of research findings rather than providing more entomological exceptions. The Bulletin publishes high quality and original research papers, ''critiques'' and review articles concerning insects or other arthropods of economic importance in agriculture, forestry, stored products, biological control, medicine, animal health and natural resource management. The scope of papers addresses the biology, ecology, behaviour, physiology and systematics of individuals and populations, with a particular emphasis upon the major current and emerging pests of agriculture, horticulture and forestry, and vectors of human and animal diseases. This includes the interactions between species (plants, hosts for parasites, natural enemies and whole communities), novel methodological developments, including molecular biology, in an applied context. The Bulletin does not publish the results of pesticide testing or traditional taxonomic revisions.
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