Acacetin alleviates energy metabolism disorder through promoting white fat browning mediated by AC-cAMP pathway.

IF 3.7 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yanan Zhang, Qianqian Huang, Xiaowei Xiong, Tingting Yin, Sheng Chen, Wanwan Yuan, Guohua Zeng, Qiren Huang
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引用次数: 0

Abstract

Acacetin (ACA), a flavone isolated from Chinese traditional medical herbs, has numerous pharmacological activities. However, little is known about the roles in white fat browning and energy metabolism. In the present study, we investigated whether and how ACA would improve energy metabolism in vivo and in vitro. ACA (20 mg/kg) was intraperitoneally injected to the mice with obesity induced by HFD for 14 consecutive days (in vivo); differentiated 3T3-L1 adipocytes were treated with ACA (20 µmol/L and 40 µmol/L) for 24 h (in vitro). The metabolic profile, lipid accumulation, fat-browning and mitochondrial contents, and so on were respectively detected. The results in vivo showed that ACA significantly reduced the body weight and visceral adipose tissue weight, alleviated the energy metabolism disorder, and enhanced the browning-related protein expressions in adipose tissue of rats. Besides, the data in vitro revealed that ACA significantly reduced the lipid accumulation, induced the expressions of the browning-related proteins and cAMP-dependent protein kinase A (PKA), and increased the mitochondrium contents, especially enhanced the energy metabolism of adipocytes; however, treatment with beta-adrenergic receptor blocker (propranolol, Pro) or adenyl cyclase (AC) inhibitor (SQ22536, SQ) abrogated the ACA-mediated effects. The data demonstrate that ACA alleviates the energy metabolism disorder through the pro-browning effects mediated by the AC-cAMP pathway. The findings would provide the experimental foundation for ACA to prevent and treat obesity and related metabolism disorders.

Abstract Image

Acacetin通过AC-cAMP途径促进白色脂肪褐变,缓解能量代谢紊乱。
acad是一种从中草药中分离得到的黄酮,具有多种药理活性。然而,人们对白色脂肪褐变和能量代谢的作用知之甚少。在本研究中,我们研究了ACA是否以及如何改善体内和体外的能量代谢。将ACA (20 mg/kg)连续14天腹腔注射于HFD致肥胖小鼠体内;分别用ACA(20µmol/L和40µmol/L)处理分化的3T3-L1脂肪细胞24 h(体外)。分别检测代谢谱、脂质积累、脂肪褐变和线粒体含量等。体内实验结果显示,ACA显著降低大鼠体重和内脏脂肪组织重量,缓解能量代谢紊乱,提高脂肪组织褐化相关蛋白表达。此外,体外实验数据显示,ACA显著降低了脂肪积累,诱导褐变相关蛋白和camp依赖性蛋白激酶A (PKA)的表达,增加了线粒体含量,特别是增强了脂肪细胞的能量代谢;然而,用β -肾上腺素受体阻滞剂(心得安,Pro)或腺苷酸环化酶(AC)抑制剂(SQ22536, SQ)治疗可消除aca介导的作用。数据表明,ACA通过AC-cAMP通路介导的促褐变作用缓解能量代谢紊乱。研究结果将为ACA预防和治疗肥胖及相关代谢紊乱提供实验基础。
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来源期刊
Journal of physiology and biochemistry
Journal of physiology and biochemistry 生物-生化与分子生物学
CiteScore
6.60
自引率
0.00%
发文量
86
审稿时长
6-12 weeks
期刊介绍: The Journal of Physiology and Biochemistry publishes original research articles and reviews describing relevant new observations on molecular, biochemical and cellular mechanisms involved in human physiology. All areas of the physiology are covered. Special emphasis is placed on the integration of those levels in the whole-organism. The Journal of Physiology and Biochemistry also welcomes articles on molecular nutrition and metabolism studies, and works related to the genomic or proteomic bases of the physiological functions. Descriptive manuscripts about physiological/biochemical processes or clinical manuscripts will not be considered. The journal will not accept manuscripts testing effects of animal or plant extracts.
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