Qualitative and Quantitative Analyses of the Enantiomers of Nicotine and Nornicotine Employing Chiral Supercritical Fluid Chromatography.

IF 1.5 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of chromatographic science Pub Date : 2024-05-31 DOI:10.1093/chromsci/bmad048

Mehdi Ashraf-Khorassani, Thomas A Perfetti, Mike F Dube, William M Coleman, John M Ferraro, Weston J Umstead

引用次数: 0

Abstract

An optimized method employing chiral supercritical fluid chromatography with diode array UV-VIS detection has been developed for the quantitative analysis of nicotine and nornicotine enantiomer distributions. The method parameters that were optimized included: column type (stationary phases, Chiralpak IG-3), column temperature (40°C), modifier types and concentration (isopropyl alcohol, 10%), additive types and concentrations (diethylamine, 0.2%), elution times (<6 min, flow rate 3 mL/min) and resolution factor (>1.2). These optimized conditions led to nicotine and nornicotine enantiomer detection limits of ~5 ng/μL with accompanying %RSD values of <2% from the analyses of commercially available nicotine-containing formulations.

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利用手性超临界流体色谱法对尼古丁和烟碱的对映体进行定性和定量分析

针对尼古丁和去甲尼古丁对映体分布的定量分析，开发了一种采用手性超临界流体色谱和二极管阵列紫外-可见检测的优化方法。优化的方法参数包括：色谱柱类型（固定相，Chiralpak IG-3）、色谱柱温度（40°C）、调节剂类型和浓度（异丙醇，10%）、添加剂类型和浓度（二乙胺，0.2%）、洗脱时间（1.2）。在这些优化条件下，尼古丁和去甲尼古丁对映体的检测限为 ~5 ng/μL，相应的 %RSD 值为

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来源期刊

Journal of chromatographic science 化学-分析化学

CiteScore

2.90

自引率

7.70%

发文量

审稿时长

5.6 months

期刊介绍： The Journal of Chromatographic Science is devoted to the dissemination of information concerning all methods of chromatographic analysis. The standard manuscript is a description of recent original research that covers any or all phases of a specific separation problem, principle, or method. Manuscripts which have a high degree of novelty and fundamental significance to the field of separation science are particularly encouraged. It is expected the authors will clearly state in the Introduction how their method compares in some markedly new and improved way to previous published related methods. Analytical performance characteristics of new methods including sensitivity, tested limits of detection or quantification, accuracy, precision, and specificity should be provided. Manuscripts which describe a straightforward extension of a known analytical method or an application to a previously analyzed and/or uncomplicated sample matrix will not normally be reviewed favorably. Manuscripts in which mass spectrometry is the dominant analytical method and chromatography is of marked secondary importance may be declined.