Single-cell multi-omics sequencing and its application in tumor heterogeneity.

IF 2.5 3区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Briefings in Functional Genomics Pub Date : 2023-07-17 DOI:10.1093/bfgp/elad009

Yuqing Sun, Zhiyu Liu, Yue Fu, Yuwei Yang, Junru Lu, Min Pan, Tian Wen, Xueying Xie, Yunfei Bai, Qinyu Ge

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引用次数: 0

Abstract

In recent years, the emergence and development of single-cell sequencing technologies have provided unprecedented opportunities to analyze deoxyribonucleic acid, ribonucleic acid and proteins at single-cell resolution. The advancements and reduced costs of high-throughput technologies allow for parallel sequencing of multiple molecular layers from a single cell, providing a comprehensive insight into the biological state and behavioral mechanisms of cells through the integration of genomics, transcriptomics, epigenomics and proteomics information. Researchers are actively working to further improve the cost-effectiveness, stability and high-throughput capabilities of single-cell multi-omics sequencing technologies and exploring their potential in precision medicine through clinical diagnostics. This review aims to survey the cutting-edge advancements in single-cell multi-omics sequencing, summarizing the representative technologies and their applications in profiling complex diseases, with a particular focus on tumors.

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单细胞多组学测序及其在肿瘤异质性研究中的应用。

近年来，单细胞测序技术的出现和发展为在单细胞分辨率上分析脱氧核糖核酸、核糖核酸和蛋白质提供了前所未有的机会。高通量技术的进步和成本的降低使得从单个细胞中对多个分子层进行平行测序成为可能，通过整合基因组学、转录组学、表观基因组学和蛋白质组学信息，可以全面了解细胞的生物状态和行为机制。研究人员正积极致力于进一步提高单细胞多组学测序技术的成本效益、稳定性和高通量能力，并通过临床诊断探索其在精准医学中的潜力。本文综述了单细胞多组学测序的最新进展，总结了代表性技术及其在复杂疾病分析中的应用，特别是在肿瘤方面的应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Briefings in Functional Genomics BIOTECHNOLOGY & APPLIED MICROBIOLOGY-GENETICS & HEREDITY

CiteScore

6.30

自引率

2.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Briefings in Functional Genomics publishes high quality peer reviewed articles that focus on the use, development or exploitation of genomic approaches, and their application to all areas of biological research. As well as exploring thematic areas where these techniques and protocols are being used, articles review the impact that these approaches have had, or are likely to have, on their field. Subjects covered by the Journal include but are not restricted to: the identification and functional characterisation of coding and non-coding features in genomes, microarray technologies, gene expression profiling, next generation sequencing, pharmacogenomics, phenomics, SNP technologies, transgenic systems, mutation screens and genotyping. Articles range in scope and depth from the introductory level to specific details of protocols and analyses, encompassing bacterial, fungal, plant, animal and human data. The editorial board welcome the submission of review articles for publication. Essential criteria for the publication of papers is that they do not contain primary data, and that they are high quality, clearly written review articles which provide a balanced, highly informative and up to date perspective to researchers in the field of functional genomics.