Investigating the chemical pathway to the formation of a single biofilm using infrared spectroscopy

IF 5.9 Q1 MICROBIOLOGY
Amy R. Crisp , Bryn Short , Laurence Rowan , Gordon Ramage , Ihtesham U.R. Rehman , Robert D. Short , Craig Williams
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引用次数: 1

Abstract

Diagnosing biofilm infections has remained a constant challenge for the last 50 years. Existing diagnostic methods struggle to identify the biofilm phenotype. Moreover, most methods of biofilm analysis destroy the biofilm making the resultant data interpretation difficult. In this study we introduce Fourier Transform Infra-Red (FTIR) spectroscopy as a label-free, non-destructive approach to monitoring biofilm progression. We have utilised FTIR in a novel application to evaluate the chemical composition of bacterial biofilms without disrupting the biofilm architecture. S. epidermidis (RP62A) was grown onto calcium fluoride slides for periods of 30 min–96 h, before semi-drying samples for analysis. We report the discovery of a chemical marker to distinguish between planktonic and biofilm samples. The appearance of new proteins in biofilm samples of varying maturity is exemplified in the spectroscopic data, highlighting the potential of FTIR for identifying the presence and developmental stage of a single biofilm.

利用红外光谱研究形成单一生物膜的化学途径
在过去的50年里,诊断生物膜感染一直是一个挑战。现有的诊断方法难以识别生物膜表型。此外,大多数生物膜分析方法都会破坏生物膜,从而使所得数据难以解释。在这项研究中,我们介绍了傅里叶变换红外光谱(FTIR)作为一种无标记、无损的方法来监测生物膜的进展。我们已经在一个新的应用中使用FTIR来评估细菌生物膜的化学组成,而不会破坏生物膜的结构。表皮葡萄球菌(RP62A)在氟化钙载玻片上生长30分钟–96小时,然后半干燥样品进行分析。我们报告发现了一种化学标记物来区分浮游生物和生物膜样本。光谱数据举例说明了不同成熟度的生物膜样品中新蛋白质的出现,突出了FTIR在识别单个生物膜的存在和发育阶段方面的潜力。
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来源期刊
Biofilm
Biofilm MICROBIOLOGY-
CiteScore
7.50
自引率
1.50%
发文量
30
审稿时长
57 days
期刊介绍:
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