The high cross-transmission in methicillin resistant Staphylococcus aureus between healthy and patient communities.

IF 1.3 Q4 MICROBIOLOGY

Iranian Journal of Microbiology Pub Date : 2023-06-01 DOI:10.18502/ijm.v15i3.12901

Behnood Haji Sheikhzadeh, Leila Rahbarnia, Alireza Dehnad, Behrooz Naghili, Parvaneh Saffarian

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Abstract

Background and Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the main causes of high mortality and morbidity in hospitals. This study was aimed to examine virulence factors, molecular typing, and the antibiotic resistance pattern of MRSA isolates in hemodialysis patients and healthy communities. Materials and Methods: Total of 231 and 400 nasal samples were obtained from hemodialysis patients and healthy communities, respectively. Virulence factors profile was examined in two groups by PCR reaction. Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) was used as a molecular typing approach. Results: Overall, 35.49% (82/231) of hemodialysis patients were positive for S. aureus, and 47.56% (39/82) of isolates were positive for mecA. In a healthy community, 15% (60/400) of samples were positive for S. aureus, and 36.66% (22/60) were positive for mecA. The frequency of MDR was significantly higher in patients group (p-value < 0.00001). The frequency of pvl (p.value = 0.003932, P<0.05) and tsst-1 (p.value = 0.003173, p < .05) were significantly higher in patients group. The highest frequency virulence factors in healthy individuals were related to hla (68.33%, 41/60), hlb (53.33%, 32/60), and Acme/arcA (46.66%) genes. Two groups were clustered by the ERIC-PCR method into 7 clusters and 2 single isolate with a 0.74 similarity index. Based on the results, each cluster was combination with healthy and patient isolates. Conclusion: Our findings indicate a notable variation in the frequency of virulence factors between S. aureus isolates obtained from dialysis patients and the healthy community.

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耐甲氧西林金黄色葡萄球菌在健康和患者群体之间的高交叉传播。

背景与目的:耐甲氧西林金黄色葡萄球菌(MRSA)是导致医院高死亡率和发病率的主要原因之一。本研究旨在检测血液透析患者和健康社区中MRSA分离株的毒力因素、分子分型和抗生素耐药性模式。材料与方法:分别从血液透析患者和健康社区采集鼻标本231份和400份。采用PCR反应检测两组毒力因子谱。采用肠杆菌重复基因间共识(ERIC-PCR)作为分子分型方法。结果:总体而言，35.49%(82/231)的血液透析患者金黄色葡萄球菌阳性，47.56%(39/82)的血液透析患者mecA阳性。健康人群金黄色葡萄球菌检出率为15% (60/400)，mecA检出率为36.66%(22/60)。患者组MDR发生率显著高于对照组(p值< 0.00001)。pvl发生率(p值= 0.003932)、Ptsst-1发生率(p值= 0.003173,p < 0.05)显著高于患者组。健康人群中频率最高的毒力因子分别与hla(68.33%， 41/60)、hlb(53.33%， 32/60)和Acme/arcA(46.66%)基因相关。采用ERIC-PCR方法将两组菌株聚为7个聚类和2个单分离株，相似指数为0.74。根据结果，将每个聚类与健康分离株和患者分离株合并。结论:我们的研究结果表明，从透析患者和健康社区获得的金黄色葡萄球菌分离株在毒力因子的频率上存在显着差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Microbiology MICROBIOLOGY-

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.