Molecular diversity of genes related to biological rhythms (period and timeless) and insecticide resistance (Na V and ace-1) in Anopheles darlingi.

IF 2.5 4区医学 Q2 PARASITOLOGY

Memorias do Instituto Oswaldo Cruz Pub Date : 2023-01-01 DOI:10.1590/0074-02760220159

Aline Cordeiro Loureiro, Alejandra Saori Araki, Rafaela Vieira Bruno, José Bento Pereira Lima, Simone Ladeia-Andrade, Liliana Santacoloma, Ademir Jesus Martins

{"title":"Molecular diversity of genes related to biological rhythms (period and timeless) and insecticide resistance (Na V and ace-1) in Anopheles darlingi.","authors":"Aline Cordeiro Loureiro, Alejandra Saori Araki, Rafaela Vieira Bruno, José Bento Pereira Lima, Simone Ladeia-Andrade, Liliana Santacoloma, Ademir Jesus Martins","doi":"10.1590/0074-02760220159","DOIUrl":null,"url":null,"abstract":"Background: Malaria is a public health concern in the Amazonian Region, where Anopheles darlingi is the main vector of Plasmodium spp. Several studies hypothesised the existence of cryptic species in An. darlingi, considering variations in behaviour, morphological and genetic aspects. Determining their overall genetic background for vector competence, insecticide resistance, and other elements is essential to better guide strategies for malaria control.Objectives: This study aimed to evaluate the molecular diversity in genes related to behaviour and insecticide resistance, estimating genetic differentiation in An. darlingi populations from Amazonian localities in Brazil and Pacific Colombian region.Methods: We amplified, cloned and sequenced fragments of genes related to behaviour: timeless (tim) and period (per), and to insecticide resistance: voltage-gated sodium channel (Na V ) and acetylcholinesterase (ace-1) from 516 An. darlingi DNA samples from Manaus, Unini River, Jaú River and Porto Velho - Brazil, and Chocó - Colombia. We discriminated single nucleotide polymorphisms (SNPs), determined haplotypes and evaluate the phylogenetic relationship among the populations.Findings: The genes per, tim and ace-1 were more polymorphic than Na V . The classical kdr and ace-1 R mutations were not observed. Phylogenetic analyses suggested a significant differentiation between An. darlingi populations from Brazil and Colombia, except for the Na V gene. There was a geographic differentiation within Brazilian populations considering per and ace-1.Conclusions: Our results add genetic data to the discussion about polymorphisms at population levels in An. darlingi. The search for insecticide resistance-related mechanisms should be extended to more populations, especially from localities with a vector control failure scenario.","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"118 ","pages":"e220159"},"PeriodicalIF":2.5000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10337825/pdf/","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Memorias do Instituto Oswaldo Cruz","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1590/0074-02760220159","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PARASITOLOGY","Score":null,"Total":0}

引用次数: 1

Abstract

Background: Malaria is a public health concern in the Amazonian Region, where Anopheles darlingi is the main vector of Plasmodium spp. Several studies hypothesised the existence of cryptic species in An. darlingi, considering variations in behaviour, morphological and genetic aspects. Determining their overall genetic background for vector competence, insecticide resistance, and other elements is essential to better guide strategies for malaria control.

Objectives: This study aimed to evaluate the molecular diversity in genes related to behaviour and insecticide resistance, estimating genetic differentiation in An. darlingi populations from Amazonian localities in Brazil and Pacific Colombian region.

Methods: We amplified, cloned and sequenced fragments of genes related to behaviour: timeless (tim) and period (per), and to insecticide resistance: voltage-gated sodium channel (Na V ) and acetylcholinesterase (ace-1) from 516 An. darlingi DNA samples from Manaus, Unini River, Jaú River and Porto Velho - Brazil, and Chocó - Colombia. We discriminated single nucleotide polymorphisms (SNPs), determined haplotypes and evaluate the phylogenetic relationship among the populations.

Findings: The genes per, tim and ace-1 were more polymorphic than Na V . The classical kdr and ace-1 R mutations were not observed. Phylogenetic analyses suggested a significant differentiation between An. darlingi populations from Brazil and Colombia, except for the Na V gene. There was a geographic differentiation within Brazilian populations considering per and ace-1.

Conclusions: Our results add genetic data to the discussion about polymorphisms at population levels in An. darlingi. The search for insecticide resistance-related mechanisms should be extended to more populations, especially from localities with a vector control failure scenario.

Abstract Image

查看原文本刊更多论文

达林按蚊生物节律(周期和永恒)和杀虫剂抗性(Na V和ace-1)相关基因的分子多样性

背景:疟疾是亚马逊地区的一个公共卫生问题，其中达林按蚊是疟原虫的主要媒介，一些研究假设在亚马逊地区存在隐种。达林吉，考虑行为、形态和遗传方面的变化。确定它们在媒介能力、杀虫剂抗性和其他因素方面的总体遗传背景对于更好地指导疟疾控制战略至关重要。目的:研究安家蚕行为和抗药性相关基因的分子多样性，估计安家蚕的遗传分化。产于巴西亚马逊地区和太平洋哥伦比亚地区的达林猴种群。方法:对516羊的行为相关基因片段进行扩增、克隆和测序，包括:时间(time)和周期(per)，以及杀虫剂抗性相关基因片段:电压门控钠通道(Na V)和乙酰胆碱酯酶(ace-1)。darlingi DNA样本来自马瑙斯、乌尼尼河、Jaú河和巴西的韦柳港，以及Chocó -哥伦比亚。我们区分了单核苷酸多态性(snp)，确定了单倍型，并评估了种群间的系统发育关系。结果:per、tim和ace-1基因多态性高于Na V。没有观察到经典的kdr和ace- 1r突变。系统发育分析表明，安培和安培之间存在显著差异。除了Na V基因外，其他物种都来自巴西和哥伦比亚。考虑到per和ace-1，巴西人口中存在地理差异。结论:本研究结果为探讨安属植物群体水平上的多态性提供了新的遗传数据。darlingi。对杀虫剂抗药性相关机制的研究应扩大到更多的人群，特别是在病媒控制失败的地区。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Memorias do Instituto Oswaldo Cruz 医学-寄生虫学

CiteScore

5.00

自引率

3.60%

发文量

审稿时长

3-8 weeks

期刊介绍： Memórias do Instituto Oswaldo Cruz is a journal specialized in microbes & their vectors causing human infections. This means that we accept manuscripts covering multidisciplinary approaches and findings in the basic aspects of infectious diseases, e.g. basic in research in prokariotes, eukaryotes, and/or virus. Articles must clearly show what is the main question to be answered, the hypothesis raised, and the contribution given by the study. Priority is given to manuscripts reporting novel mechanisms and general findings concerning the biology of human infectious prokariotes, eukariotes or virus. Papers reporting innovative methods for diagnostics or that advance the basic research with these infectious agents are also welcome. It is important to mention what we do not publish: veterinary infectious agents research, taxonomic analysis and re-description of species, epidemiological studies or surveys or case reports and data re-analysis. Manuscripts that fall in these cases or that are considered of low priority by the journal editorial board, will be returned to the author(s) for submission to another journal.