High-Fat and High-Sucrose Diet Leads to Skeletal Muscle Loss and Bladder Dysfunction in Rat.

IF 2 Q2 UROLOGY & NEPHROLOGY

Research and Reports in Urology Pub Date : 2023-01-01 DOI:10.2147/RRU.S406808

Naoki Wada, Noriyuki Abe, Kotona Miyauchi, Shogo Makino, Hidehiro Kakizaki

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Abstract

Purpose: In this study, we investigated skeletal muscle loss and bladder dysfunction caused by high-fat/high-sucrose (HFS) diet.

Methods: Twelve-week-old Sprague-Dawley (SD) female rats were fed on normal (Group N) or HFS (Group HFS) diet for 12 weeks. We conducted urodynamic investigation and pharmacologic in vitro. In addition, we measured gastrocnemius and tibialis muscle weight and protein concentration. The hypoxia-inducible factor (HIF)-1α and 8-hydroxy-2'-deoxyguanosine (8-OHdG) in the bladder were assayed.

Results: The urodynamic investigations revealed the significantly shorter intercontraction intervals and lower maximal voiding pressure in Group HFS than in Group N. Furthermore, the absolute and relative weights of the gastrocnemius muscle were found to be significantly lower in Group HFS than in Group N. The protein concentration of the gastrocnemius muscle was also significantly lower in Group HFS than in Group N. The absolute and relative weights of the bladder were also significantly lower in Group HFS than in Group N. The contractile responses of the bladder strips to electrical field stimulation and carbachol were significantly lower in Group HFS than in Group N. The HIF1α and 8OHdG in the bladder muscle were significantly higher in Group HFS than in Group N. The HFS diet reduced bladder capacity and contractility along with the loss of the gastrocnemius muscle.

Conclusion: HFS diet promotes bladder dysfunction similar to detrusor hyperreflexia with impaired contractility.

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高脂高糖饮食导致大鼠骨骼肌损失和膀胱功能障碍。

目的:本研究探讨高脂高糖饮食引起的骨骼肌萎缩和膀胱功能障碍。方法:12周龄Sprague-Dawley (SD)雌性大鼠分别饲喂正常(N组)和HFS (HFS组)饲料12周。我们进行了尿动力学和体外药理学研究。此外，我们测量腓肠肌和胫骨肌的重量和蛋白质浓度。测定膀胱缺氧诱导因子(HIF)-1α和8-羟基-2′-脱氧鸟苷(8-OHdG)水平。结果:尿动力学检查显示，HFS组间缩间隔明显短于n组，最大排尿压力明显低于n组。腓肠肌肌的绝对和相对权重被发现在HFS组明显低于N组腓肠肌肌肉的蛋白质浓度在HFS组也显著低于N组膀胱的绝对和相对权重在HFS组也明显低于N组膀胱条的收缩反应电场刺激和碳酰胆碱组在HFS组明显低于N。HFS组大鼠膀胱肌肉中HIF1α和8OHdG含量显著高于n组。HFS组大鼠膀胱容量和收缩力下降，腓肠肌萎缩。结论:HFS饮食促进膀胱功能障碍，类似于逼尿肌反射亢进，并伴有收缩功能受损。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Research and Reports in Urology UROLOGY & NEPHROLOGY-

CiteScore

3.40

自引率

0.00%

发文量

审稿时长

16 weeks

期刊介绍： Research and Reports in Urology is an international, peer-reviewed, open access, online journal. Publishing original research, reports, editorials, reviews and commentaries on all aspects of adult and pediatric urology in the clinic and laboratory including the following topics: Pathology, pathophysiology of urological disease Investigation and treatment of urological disease Pharmacology of drugs used for the treatment of urological disease Although the main focus of the journal is to publish research and clinical results in humans; preclinical, animal and in vitro studies will be published where they will shed light on disease processes and potential new therapies. Issues of patient safety and quality of care will also be considered.