Quantitative 4D imaging of biomechanical regulation of ventricular growth and maturation

IF 4.7 3区 工程技术 Q2 ENGINEERING, BIOMEDICAL
Jae Min Cho , Mong Lung Steve Poon , Enbo Zhu , Jing Wang , Jonathan T. Butcher , Tzung Hsiai
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Abstract

Abnormal cardiac development is intimately associated with congenital heart disease. During development, a sponge-like network of muscle fibers in the endocardium, known as trabeculation, becomes compacted. Biomechanical forces regulate myocardial differentiation and proliferation to form trabeculation, while the molecular mechanism is still enigmatic. Biomechanical forces, including intracardiac hemodynamic flow and myocardial contractile force, activate a host of molecular signaling pathways to mediate cardiac morphogenesis. While mechanotransduction pathways to initiate ventricular trabeculation is well studied, deciphering the relative importance of hemodynamic shear vs. mechanical contractile forces to modulate the transition from trabeculation to compaction requires advanced imaging tools and genetically tractable animal models. For these reasons, the advent of 4D multi-scale light-sheet imaging and complementary multiplex live imaging via micro-CT in the beating zebrafish heart and live chick embryos, respectively. Thus, this review highlights the complementary animal models and advanced imaging needed to elucidate the mechanotransduction underlying cardiac ventricular development.

心室生长和成熟生物力学调控的定量4D成像
心脏发育异常与先天性心脏病密切相关。在发育过程中,心内膜中的海绵状肌纤维网络,即小梁形成,变得紧密。生物力学力调节心肌分化和增殖形成小梁,但其分子机制尚不清楚。生物力学力,包括心内血流动力学和心肌收缩力,激活了许多分子信号通路来介导心脏形态发生。虽然启动心室小梁化的机械传导途径已经得到了很好的研究,但要想弄清血液动力学剪切力与机械收缩力在调节从小梁化到致密化转变方面的相对重要性,需要先进的成像工具和遗传上可操纵的动物模型。由于这些原因,分别在跳动的斑马鱼心脏和活鸡胚中出现了4D多尺度光片成像和通过微CT的互补多重活体成像。因此,这篇综述强调了阐明心室发育背后的机械转导所需的补充动物模型和先进成像。
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来源期刊
Current Opinion in Biomedical Engineering
Current Opinion in Biomedical Engineering Medicine-Medicine (miscellaneous)
CiteScore
8.60
自引率
2.60%
发文量
59
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