[The rapid ELISA method for detection of orthopoxviruses].

Q3 Medicine

Voprosy virusologii Pub Date : 2023-07-06 DOI:10.36233/0507-4088-178

N D Ushkalenko, A V Ersh, P V Filatov, A G Poltavchenko

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引用次数: 0

Abstract

Introduction: Following the successful eradication of smallpox, mass vaccination against this disease was discontinued in 1980. The unvaccinated population continues to be at risk of infection due to military use of variola virus or exposure to monkeypox virus in Africa and non-endemic areas. In cases of these diseases, rapid diagnosis is of great importance, since the promptness and effectiveness of therapeutic and quarantine measures depend on it. The aim of work is to develop a kit of reagents for enzyme-linked immunosorbent assay (ELISA) for fast and highly sensitive detection of orthopoxviruses (OPV) in clinical samples.

Materials and methods: The efficiency of virus detection was evaluated by single-stage ELISA in the cryolisate of CV-1 cell culture samples infected with vaccinia, cowpox, rabbitpox, and ectromelia viruses, as well as in clinical samples of infected rabbits and mice.

Results: The method of rapid ELISA was shown to allow the detection of OPV in crude viral samples in the range of 5.0 1025.0 103 PFU/ml, and in clinical samples with a viral load exceeding 5 103 PFU/ml.

Conclusions: The assay involves a minimum number of operations and can be performed within 45 minutes, which makes it possible to use it in conditions of a high level of biosecurity. Rapid ELISA method was developed using polyclonal antibodies, which significantly simplifies and reduces the cost of manufacturing a diagnostic system.

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ELISA快速检测正痘病毒的方法。

在成功消灭天花之后，1980年停止了针对该疾病的大规模疫苗接种。由于军事使用天花病毒或在非洲和非流行地区接触猴痘病毒，未接种疫苗的人口继续面临感染风险。在这些疾病的情况下，快速诊断非常重要，因为治疗和检疫措施的及时性和有效性取决于它。工作的目的是开发一套酶联免疫吸附试验(ELISA)试剂，用于快速和高灵敏度地检测临床样品中的正痘病毒(OPV)。材料与方法:采用单级ELISA法对牛痘、牛痘、兔痘、羊痘病毒感染的CV-1细胞培养样品的冰晶液以及感染兔、小鼠的临床样品进行病毒检测。结果:该方法可在5.0 ~ 25.0 ~ 103 PFU/ml的粗病毒样本和5 ~ 103 PFU/ml以上的临床样本中检测到OPV。结论:该检测方法操作次数最少，可在45分钟内完成，可在生物安全水平较高的条件下使用。利用多克隆抗体开发了快速ELISA方法，大大简化了诊断系统的制造过程，降低了成本。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Voprosy virusologii Medicine-Infectious Diseases

CiteScore

2.00

自引率

0.00%

发文量

期刊介绍： The journal deals with advances in virology in Russia and abroad. It publishes papers dealing with investigations of viral diseases of man, animals and plants, the results of experimental research on different problems of general and special virology. The journal publishes materials are which promote introduction into practice of the achievements of the virological science in the eradication and incidence reduction of infectious diseases, as well as their diagnosis, treatment and prevention. The reader will find a description of new methods of investigation, new apparatus and devices.