Characterization and the comprehensive expression analysis of tobacco valine-glutamine genes in response to trichomes development and stress tolerance.

IF 3.4 3区生物学 Q1 Agricultural and Biological Sciences

Botanical Studies Pub Date : 2023-07-10 DOI:10.1186/s40529-023-00376-x

Xiaoxiao Yan, Rui Luo, Xiangyang Liu, Zihang Hou, Wenyi Pei, Wenqi Zhu, Hong Cui

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引用次数: 0

Abstract

Valine-glutamine genes (VQ) acted as transcription regulators and played the important roles in plant growth and development, and stress tolerance through interacting with transcription factors and other co-regulators. In this study, sixty-one VQ genes containing the FxxxVQxxTG motif were identified and updated in the Nicotiana tobacum genome. Phylogenetic analysis indicated that NtVQ genes were divided into seven groups and genes of each group had highly conserved exon-intron structure. Expression patterns analysis firstly showed that NtVQ genes expressed individually in different tobacco tissues including mixed-trichome (mT), glandular-trichome (gT), and nonglandular-trichome (nT), and the expression levels were also distinguishing in response to methyl jasmonate (MeJA), salicylic acid (SA), gibberellic acid (GA), ethylene (ETH), high salinity and PEG stresses. Besides, only NtVQ17 of its gene family was verified to have acquired autoactivating activity. This work will not only lead a foundation on revealing the functions of NtVQ genes in tobacco trichomes but also provided references to VQ genes related stress tolerance research in more crops.

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烟草毛状体发育和胁迫耐受性中缬氨酸-谷氨酰胺基因的特征及综合表达分析。

缬氨酸-谷氨酰胺基因(Valine-glutamine genes, VQ)作为转录调控因子，通过与转录因子及其他协同调控因子相互作用，在植物的生长发育和胁迫耐受中发挥重要作用。本研究在烟草基因组中鉴定并更新了61个含有FxxxVQxxTG基序的VQ基因。系统发育分析表明，NtVQ基因可分为7组，每组基因均具有高度保守的外显子-内含子结构。表达模式分析首先表明，NtVQ基因在混合毛状体(mT)、腺状-毛状体(gT)和非腺状-毛状体(nT)等不同烟草组织中均有单独表达，且在茉莉酸甲酯(MeJA)、水杨酸(SA)、赤霉素酸(GA)、乙烯(ETH)、高盐度和PEG胁迫下表达水平也存在差异。此外，该基因家族中只有NtVQ17被证实具有自激活活性。本研究不仅将为揭示烟草毛状体中NtVQ基因的功能奠定基础，而且为更多作物中与VQ基因相关的抗逆性研究提供参考。

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来源期刊

Botanical Studies 生物-植物科学

CiteScore

5.50

自引率

2.90%

发文量

审稿时长

2.4 months

期刊介绍： Botanical Studies is an open access journal that encompasses all aspects of botany, including but not limited to taxonomy, morphology, development, genetics, evolution, reproduction, systematics, and biodiversity of all plant groups, algae, and fungi. The journal is affiliated with the Institute of Plant and Microbial Biology, Academia Sinica, Taiwan.