Electroacupuncture relieves visceral hypersensitivity through modulation of the endogenous cannabinoid system.

IF 2.4 3区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Acupuncture in Medicine Pub Date : 2023-08-01 DOI:10.1177/09645284221107699

Ning Ma, Xiaojing Li, Qiuhua Li, Diqi Yang, Shen Zhuang, Sha Nan, Ai Liu, Mingxing Ding, Yi Ding

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引用次数: 1

Abstract

Background: Electroacupuncture (EA) can effectively relieve visceral hypersensitivity (VH). However, its mechanisms are still unclear.

Objective: To investigate the impact of EA on VH caused by ileitis, and whether EA relieves VH by modulating the endogenous cannabinoid system (ECS).

Methods: Thirty male native goats were randomly divided into a saline-treated control group (Saline, n = 9) and three 2,4,6-trinitro-benzenesulfonic acid (TNBS)-treated VH model groups that underwent injection of TNBS into the ileal wall to induce VH and remained untreated (TNBS, n = 9) or received six sessions of EA (for 30 min every 3 days) (TNBS + EA, n = 6) or sham acupuncture (TNBS + Sham, n = 6). The visceromotor response (VMR) to colorectal distention (CRD) was measured after each EA treatment. Three goats in the Saline/TNBS groups were euthanized after 7 days for histopathological examination; the remaining 24 (n = 6/group) underwent sampling of the ileal wall, T11 spinal cord and brain nuclei/areas related to visceral regulation and ascending pain modulation system on day 22. Expression of cannabinoid receptor 1 (CB1R), fatty acid amide hydrolase (FAAH) and monoacylglycerol lipase (MAGL) was detected by immunohistochemistry.

Results: VMR to CRD was greater in TNBS-treated goats than in saline-treated goats (p < 0.01) from day 7 to 22. After day 7, EA-treated goats showed a decreased (p < 0.05) VMR compared with untreated TNBS-exposed goats. TNBS treatment decreased CB1R and increased FAAH and MAGL expression in the ileum and related nuclei/areas; this was reversed by EA.

Conclusion: EA ameliorates VH, probably by regulating the ECS in the intestine and nuclei/areas related to visceral regulation and descending pain modulation systems.

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电针通过调节内源性大麻素系统减轻内脏过敏。

背景:电针(EA)能有效缓解内脏超敏反应(VH)。然而，其机制尚不清楚。目的:探讨EA对回肠炎致VH的影响，以及EA是否通过调节内源性大麻素系统(ECS)来缓解VH。方法:三十男性本地山羊被随机分为saline-treated对照组(生理盐水,n = 9)和三个2,4,6-trinitro-benzenesulfonic酸(TNBS)治疗VH模型组进行了注入TNBS到回肠墙诱导VH,未经处理(TNBS, n = 9)或收到了六个交易日的EA(每3天30分钟)(TNBS + EA, n = 6)或针灸(TNBS +骗局,n = 6)。内脏运动的响应(VMR)结直肠扩张(CRD)测量每次EA后治疗。生理盐水/TNBS组3只山羊于7 d后安乐死，进行组织病理学检查;其余24只(n = 6/组)在第22天对回肠壁、T11脊髓和与内脏调节和上升疼痛调节系统相关的脑核/区域进行采样。免疫组化法检测大麻素受体1 (CB1R)、脂肪酸酰胺水解酶(FAAH)和单酰基甘油脂肪酶(MAGL)的表达。结果:第7 ~ 22天，tnbs处理山羊的VMR对CRD的影响显著高于盐水处理山羊(p < 0.01)。第7天，ea处理山羊的VMR较未处理的tnbs暴露山羊降低(p < 0.05)。TNBS治疗降低了回肠及相关核区CB1R的表达，增加了FAAH和MAGL的表达;结论:EA改善了VH，可能是通过调节肠内ECS和与内脏调节和下行疼痛调节系统相关的核/区域。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acupuncture in Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

4.70

自引率

4.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Acupuncture in Medicine aims to promote the scientific understanding of acupuncture and related treatments by publishing scientific investigations of their effectiveness and modes of action as well as articles on their use in health services and clinical practice. Acupuncture in Medicine uses the Western understanding of neurophysiology and anatomy to interpret the effects of acupuncture.