Prevalence of amyloid in ligamentum flavum of patients with lumbar spinal stenosis.

IF 5.2 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Francesco Marchi, Chiara Kessler, Daniela Distefano, Lodovico Terzi di Bergamo, Luca Fumagalli, Manuela Averaimo, Emanuele Crupi, Fabio Bergamini, Giorgia Melli, Georg Stussi, Davide Rossi, Claudio Gobbi, Paolo Ripellino, Emanuele Pravatà, Dominique E Kuhlen, Christoph Röcken, Pietro Scarone, Bernhard Gerber, Adalgisa Condoluci
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引用次数: 0

Abstract

Background: Transthyretin (ATTR) amyloidosis is often diagnosed in an advanced stage, when irreversible cardiac damage has occurred. Lumbar spinal stenosis (LSS) may precede cardiac ATTR amyloidosis by many years, offering the opportunity to detect ATTR already at the time of LSS surgery. We prospectively assessed the prevalence of ATTR in the ligamentum flavum by tissue biopsy in patients aged >50 years undergoing surgery for LSS.

Methods: Ligamentum flavum thickness was assessed pre-operatively on axial T2 magnetic resonance imaging (MRI) slices. Tissue samples from ligamentum flavum were screened centrally by Congo red staining and immunohistochemistry (IHC).

Results: Amyloid in the ligamentum flavum was detected in 74/94 patients (78.7%). IHC revealed ATTR in 61 (64.9%), whereas amyloid subtyping was inconclusive in 13 (13.8%). Mean thickness of ligamentum flavum was significantly higher at all levels in patients with amyloid (p < .05). Patients with amyloid deposits were older (73.1 ± 9.2 vs. 64.6 ± 10.1 years, p = .01). No differences in sex, comorbidities, previous surgery for carpal tunnel syndrome or LSS were observed.

Conclusions: Amyloid, mostly of the ATTR subtype, was found in four out of five patients with LSS and is associated with age and ligamentum flavum thickness. Histopathological work-up of ligamentum flavum might inform future decision making.

腰椎管狭窄症患者黄韧带淀粉样蛋白的患病率。
背景:转甲状腺素(ATTR)淀粉样变通常在发生不可逆心脏损伤的晚期诊断出来。腰椎管狭窄(LSS)可能早于心脏ATTR淀粉样变多年,这为在LSS手术时检测ATTR提供了机会。我们通过组织活检前瞻性地评估了年龄>50岁的LSS手术患者黄韧带ATTR的患病率。方法:术前采用轴向T2磁共振成像(MRI)片评估黄韧带厚度。采用刚果红染色和免疫组化(IHC)对黄韧带组织样本进行集中筛选。结果:94例患者中有74例(78.7%)检出黄韧带淀粉样蛋白。61例(64.9%)IHC显示ATTR, 13例(13.8%)淀粉样蛋白分型不确定。淀粉样蛋白患者黄韧带的平均厚度在所有水平上均显著增高(p p = 0.01)。性别、合并症、既往腕管综合征或LSS手术均无差异。结论:淀粉样蛋白主要为ATTR亚型,在5例LSS患者中发现4例,并与年龄和黄韧带厚度相关。黄韧带的组织病理学检查可能为未来的决策提供信息。
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来源期刊
Amyloid-Journal of Protein Folding Disorders
Amyloid-Journal of Protein Folding Disorders 生物-生化与分子生物学
CiteScore
10.60
自引率
10.90%
发文量
48
审稿时长
6-12 weeks
期刊介绍: Amyloid: the Journal of Protein Folding Disorders is dedicated to the study of all aspects of the protein groups and associated disorders that are classified as the amyloidoses as well as other disorders associated with abnormal protein folding. The journals major focus points are: etiology, pathogenesis, histopathology, chemical structure, nature of fibrillogenesis; whilst also publishing papers on the basic and chemical genetic aspects of many of these disorders. Amyloid is recognised as one of the leading publications on amyloid protein classifications and the associated disorders, as well as clinical studies on all aspects of amyloid related neurodegenerative diseases and major clinical studies on inherited amyloidosis, especially those related to transthyretin. The Journal also publishes book reviews, meeting reports, editorials, thesis abstracts, review articles and symposia in the various areas listed above.
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