In-vitro symbiotic germination of seeds of five mycoheterotrophic Gastrodia orchids with Mycena and Marasmiaceae fungi.

IF 1.5 4区生物学 Q4 MYCOLOGY

Mycoscience Pub Date : 2022-01-01 DOI:10.47371/mycosci.2022.04.001

Michiru Kitahara, Kosuke Nagamune, Akihiko Kinoshita, Chigusa Yugeta, Naoto Ohara, Akane Shimazaki, Yumi Yamashita, Tomohisa Yukawa, Naoki Endo, Yuki Ogura-Tsujita

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引用次数: 0

Abstract

We performed in-vitro germination tests on seeds from five Gastrodia orchids (G. confusa, G. elata var. elata, G. elata var. pallens, G. nipponica, and G. pubilabiata) using one Marasmiaceae and two Mycena isolates. Mycena sp. 1 promoted germination of all five Gastrodia orchids, with root and/or tuber formation observed in G. confusa, G. nipponica, and G. pubilabiata. No additional growth was observed in the other two orchids. Mycena sp. 2 induced G. confusa, G. elata var. elata, and G. nipponica germination, whereas Marasmiaceae sp. 1 induced G. nipponica and G. pubilabiata germination. Phylogenetic analyses indicated that the two Mycena isolates represent distinct lineages within the Mycenaceae. Mycena sp. 1 and Marasmiaceae sp. 1 are closely related to Mycena abramsii and Marasmiellus rhizomorphogenus, respectively. Our results imply that Mycena and marasmioid fungi play important roles in early development in Gastrodia species, and that Mycena fungi in particular may be common mycobionts of Gastrodia species. Root and/or tuber development was observed with four plant-fungus combinations, implying that these associations persist throughout the life cycle, whereas G. elata var. elata may require different associates over time. Our findings will contribute to elucidating the mycorrhizal associations of mycoheterotrophic orchids throughout their life cycle.

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五种真菌异养天麻兰科植物种子与迈锡纳科和马拉斯科真菌的体外共生萌发。

我们对五种天麻(conusa、elata变种、elata变种、nipponica和pubilabiata)的种子进行了体外萌发试验，使用一种Marasmiaceae和两种Mycena分离株。Mycena sp. 1能促进5种天麻的萌发，在G. confusa、G. nipponica和G. pubilabiata中均能形成根和/或块茎。在另外两种兰花中没有观察到额外的生长。Mycena sp. 2可以诱导野田葵、野田葵变种和日本野田葵的萌发，而Marasmiaceae sp. 1可以诱导日本野田葵和日本野田葵的萌发。系统发育分析表明，这两株菌丝体在菌丝体科中具有不同的谱系。Mycena sp. 1和Marasmiaceae sp. 1分别与Mycena abramsii和Marasmiellus rhizzomorphogenus亲缘关系较近。我们的研究结果表明，迈锡纳真菌和类麻菌在天麻的早期发育中起着重要的作用，迈锡纳真菌可能是天麻的常见真菌。在四种植物-真菌组合中观察到根和/或块茎的发育，这意味着这些关联在整个生命周期中持续存在，而elata var. elata可能随着时间的推移需要不同的关联。我们的发现将有助于阐明异养真菌兰花在其整个生命周期中的菌根关联。

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来源期刊

Mycoscience MYCOLOGY-

CiteScore

3.00

自引率

7.10%

发文量

审稿时长

3 months

期刊介绍： Mycoscience is the official English-language journal of the Mycological Society of Japan and is issued bimonthly. Mycoscience publishes original research articles and reviews on various topics related to fungi including yeasts and other organisms that have traditionally been studied by mycologists. The research areas covered by Mycoscience extend from such purely scientific fields as systematics, evolution, phylogeny, morphology, ecology, physiology, biochemistry, genetics, and molecular biology, to agricultural, medical, and industrial applications. New and improved applications of well-established mycological techniques and methods are also covered.