Prospective of Indole-3-Acteic Acid (IAA) and Endophytic Microbe Bacillus subtilis Strain SSA4 in Paddy Seedlings Development and Ascorbate-Glutathione (AsA-GSH) Cycle Regulation to Mitigate NaCl Toxicity.

IF 2.5 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Biotechnology Pub Date : 2025-08-01 Epub Date: 2023-04-23 DOI:10.1007/s12033-023-00743-w

Shobhit Raj Vimal, Jay Shankar Singh, Sheo Mohan Prasad

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引用次数: 0

Abstract

Plant growth promoting endophytes significantly affected plant health. The present study demonstrates effect of endophytic isolate Bacillus subtilis strain SSA4 and exogenous Indole-3-acetic acid (IAA) on paddy seedlings growth parameters, photosynthetic pigments, photosynthesis, leaf gas exchange parameters, respiration, oxidative stress biomarkers and Ascorbate-Glutathione (AsA-GSH) cycle under different NaCl (0-300 mM) stresses. The Bacillus subtilis SSA4 was identified by 16S r-RNA gene sequence analyses and NCBI BLASTn tools. The B. subtilis SSA4 tolerated 1100 mM NaCl and produced IAA (42.15 µg m/L) at 300 mM NaCl stress. The paddy genotype (HUR 917) treated with exogenous IAA (21 µg m/L) and B. subtilis strain SSA4 egg cell based bioformulation was significantly affected seedlings physiology and biochemistry at lower (150 mM) and higher (300 mM) NaCl doses. In conclusion, co-inoculation found as effective green tool to mitigating salinity stress in paddy seedlings.

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吲哚-3-乙酸（IAA）和内生微生物枯草芽孢杆菌SSA4在水稻幼苗发育和抗坏血酸-谷胱甘肽（AsA-GSH）循环调控中减轻NaCl毒性的前景

植物生长促进内生菌对植物健康有显著影响。本研究研究了不同NaCl （0-300 mM）胁迫下，内生枯草芽孢杆菌SSA4和外源吲哚-3-乙酸（IAA）对水稻幼苗生长参数、光合色素、光合作用、叶片气体交换参数、呼吸、氧化应激生物标志物和抗坏血酸-谷胱甘肽（AsA-GSH）循环的影响。采用16S r-RNA基因序列分析和NCBI BLASTn工具鉴定枯草芽孢杆菌SSA4。枯草芽孢杆菌SSA4耐1100 mM NaCl，在300 mM NaCl胁迫下产生42.15µg m/L的IAA。外源IAA（21µg /L）和枯草芽孢杆菌菌株SSA4卵细胞生物制剂对水稻基因型（HUR 917）在低（150 mM）和高（300 mM） NaCl处理下的幼苗生理生化有显著影响。综上所述，共接种是缓解水稻幼苗盐度胁迫的有效绿色工具。

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来源期刊

Molecular Biotechnology 医学-生化与分子生物学

CiteScore

4.10

自引率

3.80%

发文量

165

审稿时长

6 months

期刊介绍： Molecular Biotechnology publishes original research papers on the application of molecular biology to both basic and applied research in the field of biotechnology. Particular areas of interest include the following: stability and expression of cloned gene products, cell transformation, gene cloning systems and the production of recombinant proteins, protein purification and analysis, transgenic species, developmental biology, mutation analysis, the applications of DNA fingerprinting, RNA interference, and PCR technology, microarray technology, proteomics, mass spectrometry, bioinformatics, plant molecular biology, microbial genetics, gene probes and the diagnosis of disease, pharmaceutical and health care products, therapeutic agents, vaccines, gene targeting, gene therapy, stem cell technology and tissue engineering, antisense technology, protein engineering and enzyme technology, monoclonal antibodies, glycobiology and glycomics, and agricultural biotechnology.