Direct Organogenesis of Citrus Cultivars from Shoot Tip Nodal Segments.

Q3 Biochemistry, Genetics and Molecular Biology
Zekeria Yusuf, Mulugeta Desta, Wassu Mohammed
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引用次数: 0

Abstract

Background: Citrus cultivar improvement via conventional breeding strategies is impeded by factors related to its reproductive biology. The orange is a hybrid between pomelo (Citrus maxima) and mandarin (Citrus reticulata). Among various orange cultivars, Valencia oranges have a bit of bitter tang mixed in with their sweetness, as Navel oranges are, the most widely cultivated citrus species, quite sweeter, and also don't contain any seeds. Tangelo mandarin orange cultivar is a hybrid of C. reticulata × C. maxima or × C. paradisi.

Objective: The present study was undertaken to optimize the hormonal composition of the media with regard to plant growth regulators for in vitro propagation of sweet orange cultivars from nodal segment explants.

Methods: The nodal segment explants were collected from three citrus cultivars, Washington Navel, Valencia and Tangelo. Murashige and Skoog (MS) medium supplemented with sucrose and different concentrations of growth regulators was used for shoot proliferation and root induction, and the optimum medium composition was assessed. The patent for Citrus Tissue Culture was obtained from the Office of Research Affairs, Haramaya University.

Results: The results indicate that the highest shoot response was recorded for Washington's navel with maximum shoot proliferation rate (99.75%), shoot number per explant (1.76), shoot length (10.70 cm), leaf number per explants (3.54) after three weeks of culture. In all experiments, no growth was observed for the basal MS medium. Phytohormone combinations of IAA (1.2 mg/L) and kinetin (2.0 mg/L) were found to be the best for shoot proliferation. Among the cultivars, there were significant differences for the highest rooting rate (81.255), root number (2.22), and root length (2.95 cm) variables for Washington Navel. The lowest rooting rate (48.45%), root number (1.47) and root length (2.26 cm) were observed for Valencia. The highest rooting rate (84.90%), root number per microshoot (2.22) and root length (3.05 cm) was on MS medium supplemented with 1.5 mg/L NAA.

Conclusion: A comparison of different concentrations of IAA and NAA on root induction of microshoots from nodal segments of citrus cultivars demonstrated NAA was a more effective hormone than IAA.

柑橘栽培品种的芽尖节段直接器官发生。
背景:通过传统育种策略改良柑橘栽培品种受到其生殖生物学相关因素的阻碍。橙子是柚子(Citrus maxima)和柑橘(Citrus reticulata)的杂交种。在各种橙子栽培品种中,瓦伦西亚橙子的甜味中夹杂着一点苦味,而脐橙是栽培最广泛的柑橘品种,甜度较高,而且不含任何种子。柚子柑栽培品种是 C. reticulata × C. maxima 或 × C. paradisi 的杂交种:本研究旨在优化从节段外植体体外繁殖甜橙栽培品种的培养基中植物生长调节剂的激素组成:方法:从华盛顿脐橙、瓦伦西亚和丹桂三种柑橘栽培品种中采集节段外植体。使用添加了蔗糖和不同浓度生长调节剂的 Murashige 和 Skoog(MS)培养基进行芽增殖和根诱导,并评估了最佳培养基成分。柑橘组织培养的专利权由原谷大学研究事务办公室授予:结果表明,华盛顿脐橙的芽反应最高,培养三周后,芽增殖率(99.75%)、每个外植体的芽数(1.76)、芽长(10.70 厘米)和每个外植体的叶片数(3.54)均达到最高值。在所有实验中,基础 MS 培养基均未观察到生长。IAA(1.2 毫克/升)和松香素(2.0 毫克/升)的植物激素组合对嫩芽增殖效果最好。在各栽培品种中,华盛顿脐橙的最高生根率(81.255)、根数(2.22)和根长(2.95 厘米)变量存在显著差异。瓦伦西亚的生根率(48.45%)、根数(1.47)和根长(2.26 厘米)最低。在添加了 1.5 毫克/升 NAA 的 MS 培养基上,华盛顿脐橙的生根率(84.90%)、每小芽生根数(2.22)和根长(3.05 厘米)最高:比较了不同浓度的 IAA 和 NAA 对柑橘栽培品种节段小芽生根诱导的影响,结果表明 NAA 是比 IAA 更有效的激素。
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来源期刊
Recent patents on biotechnology
Recent patents on biotechnology Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
2.90
自引率
0.00%
发文量
51
期刊介绍: Recent Patents on Biotechnology publishes review articles by experts on recent patents on biotechnology. A selection of important and recent patents on biotechnology is also included in the journal. The journal is essential reading for all researchers involved in all fields of biotechnology.
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