The Effect of Zonisamide on Rat Bone Mass, Structure, and Metabolism.

IF 2.9 4区医学 Q2 PHARMACOLOGY & PHARMACY

Pharmacology Pub Date : 2023-01-01 DOI:10.1159/000529970

Iva Karesova, Julius Simko, Sona Fekete, Eva Zimcikova, Jana Malakova, Helena Zivna, Ladislava Pavlikova, Vladimir Palicka

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引用次数: 0

Abstract

Introduction: Our study aimed to investigate the effect of zonisamide (ZNS) on bone metabolism in the rat model.

Methods: Eight-week-old rats were divided into four groups. The sham-operated control group (SHAM) and the control group after orchidectomy (ORX) received the standard laboratory diet (SLD). The experimental group after orchidectomy (ORX+ZNS) and the sham-operated control group (SHAM+ZNS) received SLD enriched with ZNS for 12 weeks. Bone marker concentrations in serum of receptor activator of nuclear factor kappa B ligand, PINP, and osteoprotegerin, and the levels of sclerostin and bone alkaline phosphatase in bone homogenate, were measured using an enzyme-linked immunosorbent assay. Bone mineral density (BMD) was measured by dual-energy X-ray absorptiometry. The femurs were used for biomechanical testing.

Results: We found a statistically significant reduction in BMD and biomechanical strength 12 weeks after orchidectomy of the rats (ORX). After ZNS administration to orchidectomized rats (ORX+ZNS) and the sham-operated control rats (SHAM+ZNS), there were no statistically significant changes in BMD, bone turnover markers, or biomechanical properties as compared with the ORX group and SHAM group.

Conclusions: The results suggest that administration of ZNS to rats exerts no negative effect on BMD, bone metabolism markers, or biomechanical properties.

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唑尼沙胺对大鼠骨量、结构和代谢的影响。

本研究旨在探讨唑尼沙胺(ZNS)对大鼠骨代谢的影响。方法:将8周龄大鼠分为4组。假手术对照组(SHAM)和兰花切除术后对照组(ORX)给予标准实验室饮食(SLD)。实验组(ORX+ZNS)和假手术对照组(SHAM+ZNS)给予富含ZNS的SLD治疗，持续12周。采用酶联免疫吸附法测定血清中核因子κ B配体受体激活剂、PINP和骨保护素的骨标志物浓度，以及骨匀浆中硬化蛋白和骨碱性磷酸酶的水平。采用双能x线骨密度仪测定骨密度。股骨用于生物力学测试。结果:我们发现大鼠睾丸切除术(ORX) 12周后骨密度和生物力学强度有统计学意义的降低。去兰大鼠(ORX+ZNS)和假手术对照大鼠(SHAM+ZNS)给予ZNS后，与ORX组和SHAM组相比，骨密度、骨转换标志物或生物力学性能均无统计学意义变化。结论:ZNS给药对大鼠骨密度、骨代谢指标和生物力学性能均无负面影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pharmacology 医学-药学

CiteScore

5.60

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： ''Pharmacology'' is an international forum to present and discuss current perspectives in drug research. The journal communicates research in basic and clinical pharmacology and related fields. It covers biochemical pharmacology, molecular pharmacology, immunopharmacology, drug metabolism, pharmacogenetics, analytical toxicology, neuropsychopharmacology, pharmacokinetics and clinical pharmacology. In addition to original papers and short communications of investigative findings and pharmacological profiles the journal contains reviews, comments and perspective notes; research communications of novel therapeutic agents are encouraged.