{"title":"Magnetically Controlled 3D Cartilage Regeneration.","authors":"Xia Chen, Ruhong Zhang, Qun Zhang, Zhicheng Xu, Feng Xu, Datao Li, Yiyuan Li","doi":"10.1177/19476035231183254","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>The cartilage regeneration field has not yet overcome the issue of effective \"shaping\": growing regenerated cartilage in the desired shape, and maintaining that shape, is problematic. This study reports on a new method of cartilage regeneration in which the cartilage is shaped in three dimensions. Since cartilage is composed only of cartilage cells and an abundant extracellular matrix with no blood circulation, once it is damaged, the lack of nutrient supply means that it is difficult to repair. Scaffold-free cell sheet technology plays an important role in cartilage regeneration, avoiding inflammation and immune response caused by scaffold materials. However, cartilage regenerated from the cell sheet needs to be sculpted and shaped before it can be used for cartilage defect transplantation.</p><p><strong>Design: </strong>In this study, we used a new ultra-strong magnetic-responsive Fe3O4 nanoparticle (MNP) to shape the cartilage <i>in vitro</i>. Super-magnetic Fe3O4 microspheres are manufactured by co-assembling negatively charged Cetyltrimethylammonium bromide (CTAB) and positively charged Fe3+ under solvothermal conditions.</p><p><strong>Results: </strong>The Fe3O4 MNPs are swallowed by chondrocytes, and the MNP-labeled chondrocytes are acted upon by the magnetic field. The predetermined magnetic force makes the tissues coalesce to form a multilayer cell sheet with a predetermined shape. The shaped cartilage tissue is regenerated in the transplanted body, and the nano magnetic control particles do not affect cell viability. The nanoparticles in this study improve the efficiency of cell interaction through super-magnetic modification, and to a certain extent change the way the cells absorb magnetic iron nanoparticles. This phenomenon allows a more orderly and compact alignment of the cartilage cell extracellular matrix, promotes ECM precipitation and cartilage tissue maturation, and improves the efficiency of cartilage regeneration.</p><p><strong>Conclusion: </strong>The magnetic bionic structure, which contains specific magnetic particle-labeled cells, is deposited layer by layer to generate a three-dimensional structure with repair function, and further induce the production of cartilage. This study describes a new method for the regeneration of tissue engineered cartilage which has broad application prospects in regenerative medicine.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":null,"pages":null},"PeriodicalIF":2.7000,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11418502/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"CARTILAGE","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1177/19476035231183254","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/7/4 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"ORTHOPEDICS","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: The cartilage regeneration field has not yet overcome the issue of effective "shaping": growing regenerated cartilage in the desired shape, and maintaining that shape, is problematic. This study reports on a new method of cartilage regeneration in which the cartilage is shaped in three dimensions. Since cartilage is composed only of cartilage cells and an abundant extracellular matrix with no blood circulation, once it is damaged, the lack of nutrient supply means that it is difficult to repair. Scaffold-free cell sheet technology plays an important role in cartilage regeneration, avoiding inflammation and immune response caused by scaffold materials. However, cartilage regenerated from the cell sheet needs to be sculpted and shaped before it can be used for cartilage defect transplantation.
Design: In this study, we used a new ultra-strong magnetic-responsive Fe3O4 nanoparticle (MNP) to shape the cartilage in vitro. Super-magnetic Fe3O4 microspheres are manufactured by co-assembling negatively charged Cetyltrimethylammonium bromide (CTAB) and positively charged Fe3+ under solvothermal conditions.
Results: The Fe3O4 MNPs are swallowed by chondrocytes, and the MNP-labeled chondrocytes are acted upon by the magnetic field. The predetermined magnetic force makes the tissues coalesce to form a multilayer cell sheet with a predetermined shape. The shaped cartilage tissue is regenerated in the transplanted body, and the nano magnetic control particles do not affect cell viability. The nanoparticles in this study improve the efficiency of cell interaction through super-magnetic modification, and to a certain extent change the way the cells absorb magnetic iron nanoparticles. This phenomenon allows a more orderly and compact alignment of the cartilage cell extracellular matrix, promotes ECM precipitation and cartilage tissue maturation, and improves the efficiency of cartilage regeneration.
Conclusion: The magnetic bionic structure, which contains specific magnetic particle-labeled cells, is deposited layer by layer to generate a three-dimensional structure with repair function, and further induce the production of cartilage. This study describes a new method for the regeneration of tissue engineered cartilage which has broad application prospects in regenerative medicine.
期刊介绍：
CARTILAGE publishes articles related to the musculoskeletal system with particular attention to cartilage repair, development, function, degeneration, transplantation, and rehabilitation. The journal is a forum for the exchange of ideas for the many types of researchers and clinicians involved in cartilage biology and repair. A primary objective of CARTILAGE is to foster the cross-fertilization of the findings between clinical and basic sciences throughout the various disciplines involved in cartilage repair.
The journal publishes full length original manuscripts on all types of cartilage including articular, nasal, auricular, tracheal/bronchial, and intervertebral disc fibrocartilage. Manuscripts on clinical and laboratory research are welcome. Review articles, editorials, and letters are also encouraged. The ICRS envisages CARTILAGE as a forum for the exchange of knowledge among clinicians, scientists, patients, and researchers.
The International Cartilage Repair Society (ICRS) is dedicated to promotion, encouragement, and distribution of fundamental and applied research of cartilage in order to permit a better knowledge of function and dysfunction of articular cartilage and its repair.
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