Effect and mechanism of pearl on ovarian function of rats with premature ovarian failure induced by tripterygium glycosides

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine Pub Date : 2023-07-01 DOI:10.1016/j.jtcme.2023.02.004

Siyin Han , Hongxuan Li , Rui Lu , Jiaxin Feng , Kai Tang , Sihui Li , Jiang Lin

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引用次数: 0

Abstract

Background and aim

Recent studies show that combination of apoptosis and oxidative stress forms a “vicious circle” in the process of premature ovarian failure (POF). Pearl extract has a good effect for anti-oxidation and anti-aging in vitro and vivo and can be used to treat various aging diseases. However, reports about effect and mechanism of pearl on ovarian function of premature ovarian failure (POF)are limited.

Experimental procedure

The effect and mechanism of pearl on ovarian function of rats with POF were evaluated using rats with premature ovarian failure induced by tripterygium glycosides. The estrous cycle, contents of serum reproductive hormones, tissue structure, oxidative stress level, autophagy and apoptotic protein expression, and MAPK signaling pathway of ovary were assessed to characterise pearl.

Result and conclusion

Low, medium and high-dose pearl improved the estrous cycle in POF rats, and high-dose pearl was the best in terms of recovery effect; high-dose pearl significantly increased (P < 0.05) contents of E2, AMH and GSH, activities of SOD, CAT and GSH-PX and follicular development, while significantly decreased (P < 0.05)contents of FSH, LH and ROS and MDA in POF rats; low, medium and high-dose pearl notably reduced (P < 0.05) the apoptotic protein cleaved-caspase 3 and Bax expression, and MAPK signaling pathway of ERK1/2, p38 and JNK in POF rats, among which high-dose pearl behaved best. Medium and high-dose pearl apparently raised (P < 0.05)expressions of autophagy protein LC3II, Beclin-1 and p62 in POF rats. Therefore, pearl can effectively enhance ovarian function of POF rats. The optimal concentration was found to be 740 mg kg⁻¹ at a high dose. The mechanism may be related with the enhanced follicular development through improving granulosa cell autophagy and inhibiting granulosa cell apoptosis by inhibition of MAPK signaling pathway after scavenging excessive ROS.

Section

1. Natural Products.

Taxonomy (classification by EVISE)

Ovarian Cancer, Chinese Herbal Medicine, Traditional Medicine, Oxidative Stress, Antioxidant Studies, Rat, Autophagy.

Abstract Image

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珍珠对雷公藤多苷致卵巢早衰大鼠卵巢功能的影响及机制

背景与目的最近的研究表明，细胞凋亡和氧化应激在卵巢早衰（POF）过程中形成了一个“恶性循环”。珍珠提取物具有良好的体内外抗氧化和抗衰老作用，可用于治疗各种衰老疾病。然而，关于珍珠对卵巢早衰（POF）卵巢功能的影响及其机制的报道有限。实验方法采用雷公藤多甙诱发卵巢早衰大鼠，观察珍珠对卵巢功能的影响及其机制。对珍珠的发情周期、血清生殖激素含量、组织结构、氧化应激水平、自噬和凋亡蛋白表达以及MAPK信号通路进行了评估。结果与结论低、中、高剂量珍珠对POF大鼠发情周期均有改善作用，其中高剂量珍珠的恢复效果最好；大剂量珍珠显著提高（P＜0.05）POF大鼠E2、AMH和GSH含量，SOD、CAT和GSH-PX活性和卵泡发育，同时显著降低（P＜0.01）FSH、LH和ROS及MDA含量；低、中、高剂量珍珠显著降低（P＜0.05）POF大鼠凋亡蛋白裂解的caspase 3和Bax的表达，以及ERK1/2、p38和JNK的MAPK信号通路，其中高剂量珍珠表现最好。中剂量和高剂量珍珠明显提高（P＜0.05）POF大鼠自噬蛋白LC3II、Beclin-1和p62的表达。因此，珍珠能有效增强POF大鼠的卵巢功能。高剂量时的最佳浓度为740 mg kg−1。该机制可能与清除过量ROS后通过改善颗粒细胞自噬和抑制MAPK信号通路抑制颗粒细胞凋亡来增强卵泡发育有关。天然产物。分类（EVISE分类）卵巢癌症，中草药，传统医学，氧化应激，抗氧化研究，大鼠，自噬。

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来源期刊

Journal of Traditional and Complementary Medicine Medicine-Complementary and Alternative Medicine

CiteScore

9.30

自引率

6.70%

发文量

审稿时长

66 days

期刊介绍： eJTCM is committed to publish research providing the biological and clinical grounds for using Traditional and Complementary Medical treatments as well as studies that demonstrate the pathophysiological and molecular/biochemical bases supporting the effectiveness of such treatments. Review articles are by invitation only. eJTCM is receiving an increasing amount of submission, and we need to adopt more stringent criteria to select the articles that can be considered for peer review. Note that eJTCM is striving to increase the quality and medical relevance of the publications.