An agarose-alginate microfluidic device for the study of spheroid invasion, ATRA inhibits CAFs-mediated matrix remodeling.

IF 2 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Cytotechnology Pub Date : 2023-08-01 Epub Date: 2023-04-11 DOI:10.1007/s10616-023-00578-y
Mohammad Reza Nasiraee, Shabnam Shahrivari, Soheila Sayad, Hoda Mahdavi, Neda Saraygord-Afshari, Zeinab Bagheri
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引用次数: 0

Abstract

Growing evidence demonstrates that cancer-associated fibroblasts (CAF) are responsible for tumor genesis, growth, metastasis, and treatment response. Therefore, targeting these cells may contribute to tumor control. It has been proposed that targeting key molecules and pathways of proliferative functions can be more effective than killing CAFs. In this regard, multicellular aggregates, like spheroids, can be used as human tumor models. Spheroids closely resemble human tumors and mimic many of their features. Microfluidic systems are ideal for cultivation and study of spheroids. These systems can be designed with different biological and synthetic matrices in order to have a more realistic simulation of the tumor microenvironment (TME). In this study, we investigated the effect of all-trans retinoic acid (ATRA) on 3D spheroid invasion of MDA-MB cells exposed to hydrogel matrix derived from CAFs. The number of invasive cells significantly decreased in CAF-ECM hydrogel treated with ATRA (p < 0.05), which indicates that ATRA could be effective for CAFs normalization. This experiment was done using an agarose-alginate microfluidic chip. As compared with common methods, such hydrogel casting is an easier method for chip fabrication and can even reduce costs.

Graphical abstract:

Supplementary information: The online version contains supplementary material available at 10.1007/s10616-023-00578-y.

Abstract Image

一种用于研究球体侵袭的琼脂糖-藻酸盐微流体装置,ATRA抑制CAFs介导的基质重塑。
越来越多的证据表明,癌症相关成纤维细胞(CAF)负责肿瘤的发生、生长、转移和治疗反应。因此,靶向这些细胞可能有助于肿瘤控制。有人提出,靶向增殖功能的关键分子和途径可能比杀死CAFs更有效。在这方面,多细胞聚集体,如球体,可以用作人类肿瘤模型。球体与人类肿瘤非常相似,并模仿了它们的许多特征。微流体系统是培养和研究球体的理想选择。这些系统可以用不同的生物和合成基质设计,以便对肿瘤微环境(TME)进行更真实的模拟。在本研究中,我们研究了全反式维甲酸(ATRA)对暴露于CAFs水凝胶基质的MDA-MB细胞的3D球体侵袭的影响。ATRA处理的CAF-ECM水凝胶中的侵袭细胞数量显著减少(p 图形摘要:补充信息:在线版本包含补充材料,可访问10.1007/s10616-023-00578-y。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Cytotechnology
Cytotechnology 生物-生物工程与应用微生物
CiteScore
4.10
自引率
0.00%
发文量
49
审稿时长
6-12 weeks
期刊介绍: The scope of the Journal includes: 1. The derivation, genetic modification and characterization of cell lines, genetic and phenotypic regulation, control of cellular metabolism, cell physiology and biochemistry related to cell function, performance and expression of cell products. 2. Cell culture techniques, substrates, environmental requirements and optimization, cloning, hybridization and molecular biology, including genomic and proteomic tools. 3. Cell culture systems, processes, reactors, scale-up, and industrial production. Descriptions of the design or construction of equipment, media or quality control procedures, that are ancillary to cellular research. 4. The application of animal/human cells in research in the field of stem cell research including maintenance of stemness, differentiation, genetics, and senescence, cancer research, research in immunology, as well as applications in tissue engineering and gene therapy. 5. The use of cell cultures as a substrate for bioassays, biomedical applications and in particular as a replacement for animal models.
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